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Thursday, July 18, 2019

8:00 - 18:00
Registration/Information

9:00 - 9:30
Piere Rodriguez-Aliaga, Stanford University, USA
Carlos Heeren, UTEC, Peru
Carlos Bustamante, University of California, Berkeley, USA
Opening Remarks

Session I: Molecular Motors
Shixin Liu, the Rockefeller University, USA, Chair

9:30 - 10:15
Carlos Bustamante, University of California, Berkeley, USA
Co-temporal Force and Fluorescence Measurements Reveal a Ribosomal Gear-shift Mechanism of Translation Regulation by mRNA Secondary Structures

10:15 - 10:30
Shixin Liu, the Rockefeller University, USA *
Functional Plasticity in Ring-shaped Molecular Motors

10:30 - 11:00
Coffee Break

11:00 - 11:15
Stefan Niekamp, University of California, San Francisco, USA*
Tracking Dynein Stepping Along Microtubules with Nanometer Accuracy Using Three-color Imaging

11:15 - 11:30
Juan P. Castillo, University of California, Berkeley, USA *
Challenging a Viral DNA Packaging Motor with Modified Substrates

11:30 - 11:45
Arne Gennerich, Albert Einstein College of Medicine, USA *
Molecular Mechanism of Cytoplasmic Dynein Tension Sensing

11:45 - 12:15
Flash Talks

12:15 - 14:30
Lunch (on own)

14:30 - 15:45
Poster Session I

15:45 - 16:15
Coffee Break

Session II: Nanoscale Imaging of Cellular Processes I
Lía Pietrasanta, University of Buenos Aires, Argentina, Chair

16:15 - 16:45
Jie Xiao, Johns Hopkins University, USA
Gene Regulation and Chromosome Conformation

16:45 - 17:15
Fernando Stefani, University of Buenos Aires, Argentina
Far-field Fluorescence Nanoscopy with Sub-10 nm Resolution

17:15 - 18:00
Simon Scheuring, Weill Cornell Medicine, USA
High-speed Atomic Force Microscopy: Structural Dynamics of Single Unlabeled Proteins

18:00 - 20:00
Social Event

Friday, July 19, 2019

8:00 - 18:00
Registration/Information

Session III: Nanoscale Imaging of Cellular Processes II
Edward Málaga-Trillo, Cayetano Heredia University, Peru, Chair

9:00 - 9:45
Tomas Kirchhausen, Harvard University, USA
Imaging Subcellular Dynamics from Molecules to Multicellular Organisms

9:45 - 10:15
Melike Lakadamyali, University of Pennsylvania, USA
Superresolution Imaging of Chromatin Organization

10:15 - 10:30
María Benítez-Jones, New York University, USA *
Single-Molecule Tracking of DNA Repair Factory Dynamics in Live Cells

10:30 - 10:45
Elena Kudryashova, the Ohio State University, USA *
Toxicity Amplification Mechanism of Actin Crosslinking Toxin Revealed by Single-Molecule Imaging

10:45 - 11:15
Coffee Break

Session IV: Biopolymers
Christian A.M. Wilson, University of Chile, Chile, Chair

11:15 - 11:45
Márcio Santos Rocha, Universidade Federal de Viçosa, Brazil
DNA Interactions with Drugs and Small Molecules Investigated by Single Molecule Force Spectroscopy

11:45 - 12:00
J Andres Rivas Pardo, Universidad Mayor, Chile *
The Power of the Force: Mechano-physiology of the Giant Titin

12:00 - 12:30
Flash Talks

12:30 - 14:45
Lunch (on own)

14:45 - 16:00
Poster Session II

16:00 - 16:30
Coffee Break

Session V: Protein Synthesis
Christian Kaiser, Johns Hopkins University, USA, Chair

16:30 - 16:45
Tatsuya Morisaki, Colorado State University, USA *
Visualization and Quantification of Translation Dynamics in Living Cells at Single Molecule Resolution

16:45 - 17:00
Christian Kaiser, Johns Hopkins University, USA *
The Ribosome Cooperates with Chaperones to Guide Multidomain Protein Folding

17:00 - 17:15
Scott Blanchard, Weill Cornell Medicine, USA *
Endogenous Ribosomal RNA Sequence Variation Can Modulate Stress Response Gene Expression and Phenotype

17:15 - 18:00
Ruben Gonzalez, Columbia University, USA
Ribosomes in Action: The Role of Dynamics in the Mechanism and Regulation of Translation

Saturday, July 20, 2019

8:00 - 18:00
Registration/Information

Session VI: Protein Folding and Allostery
Piere Rodriguez-Aliaga, Stanford University, USA, Chair

9:00 - 9:45
Matthias Rief, Technische Universität München, Germany
Single Molecule Mechanics of Protein Folding and Binding

9:45 - 10:15
Rodrigo Maillard, Georgetown University, USA
Activation of a Protein Kinase via Asymmetric Allosteric Coupling of Structurally Conserved Signaling Modules

10:15 - 10:45
Mauricio Baez, University of Chile, Chile
Folding Free Energy Barriers of Topologically Knotted Proteins

10:45 - 11:15
Coffee Break

Session VII: Protein Processing Machines
Rodrigo Maillard, Georgetown University, USA, Chair

11:15 - 11:45
Christian A.M. Wilson, University of Chile, Chile
Studying the Mechanical Properties of Protein Translocation by Optical Tweezers and Nanorheology

11:45 - 12:15
Piere Rodriguez-Aliaga, Stanford University, USA
A Finely Tuned Molecular Motor: Mechanochemistry and Power Efficiency in the AAA+ Protease Machine ClpXP

12:15 - 12:30
Erik Jonsson, University of California, Berkeley, USA *
Direct Observation of Substrate Translocation and Conformational Dynamics in the 26s Proteasome

12:30 - 12:45
Gabriel Lander, The Scripps Research Institute, USA *
Mechanisms of Mitochondrial Machines of Mass Destruction

12:45 - 14:45
Lunch (on own)

14:45 - 16:00
Free Time

16:00 - 16:15
Coffee Break

Session VIII: Nucleic Acids Machines
Laura Finzi, Emory University, USA, Chair

16:15 - 16:30
Laura Finzi, Emory University, USA *
Transcription through Roadblocks

16:30 - 16:45
Wei Ting (Chelsea) Lee, New York University, USA *
Superresolution Imaging of Replication-associated G4 DNA in Human Cells

16:45 - 17:00
Nikos Hatzakis, University of Copenhagen, Denmark *
Direct Observation of CRISPR-Cas12 as Conformational Sampling Reveals How Conformational Activation Promotes Catalysis and Resetting of the Endonuclease Activity

17:00 - 17:15
Ian Nova, University of Washington, USA *
Detecting Single Steps During Transcription and a Half-translocated Pause Complex of E. coli RNA Polymerase Using Nanopore Tweezers

17:15 - 18:00
Michelle Wang, Cornell University, USA
Molecular Highways – Torsional Consequences of DNA Motor Proteins

Sunday, July 21, 2019

8:30 – 12:30
Registration/Information

Session IX: Extracting Kinetics from Molecular Processes
Mauricio Baez, University of Chile, Chile, Chair

9:00;- 9:45
Gijs Wuite, Vrije Universiteit Amsterdam, the Netherlands
Single Molecule Manipulation and Imaging of Complex DNA-Protein Transactions

9:45 - 10:15
Olga Dudko, University of California, San Diego, USA
Mechanical Fingerprints of Biomolecules, Decoded

10:15 - 10:30
Sara Tafoya, LUMICKS, USA *
Using a System’s Equilibrium Behavior to Reduce Its Energy Dissipation in Non-Equilibrium Processes

10:30 - 11:00
Coffee Break

Session X: Nanoscale Imaging of Cellular Processes III
Tomas Kirchhausen, Harvard University, USA, Chair

11:00 - 11:30
Eli Rothenberg, New York University, USA
A Single-Molecule View of Mammalian DNA Double-strand Break Repair

11:30 - 12:15
Xiaowei Zhuang, Harvard University, USA
Imaging at the Genomic-scale: From 3D Organization of the Genomic DNA to Cell Atlas of Complex Tissues

12:15 - 12:30
Carlos Bustamante, University of California, Berkeley, USA
Closing Remarks and Biophysical Journal Poster Awards