Monday, May 6, 2019

16:30 – 18:30

17:30 – 19:30
Welcome Reception

Tuesday, May 7, 2019

8:00 – 18:00

8:30 – 8:40
Morten Gram Pedersen, University of Padova, Italy
Opening Remarks

Session I: Exocytosis I
Sebastian Barg, Uppsala University, Chair

8:40 – 9:10
Arun Anantharam, University of Michigan, USA
Synaptotagmin-7 Endows a Population of Chromaffin Granules with Distinct Calcium Sensing and Fusion Properties

9:10 – 9:40
Ben O'Shaughnessy, Columbia University, USA
Coarse-Grained Mathematical Modeling of Neurotransmitter Release

9:40 – 10:00
Yongli Zhang, Yale University, USA*
Sec1/Munc18 Proteins Catalyze SNARE Assembly by Templating SNARE Folding and Association 

10:00 – 10:20
Alex Kreutzberger, University of Virginia, USA*
Reconstitution of Regulated Exocytosis of Different Secretory Vesicle Types

10:20 – 10:50
Coffee Break

Session II: Systems
Dinah Loerke, University of Denver, USA, Chair

10:50 – 11:20
Jens Rettig, Saarland University, Germany
Molecular Insights Into Exo- and Endocytosis of Cytotoxic Granules

11:20 – 11:50
Tomas Kirchhausen, Harvard University, USA
Imaging Subcellular Dynamics from Molecules to Multicellular Organisms

11:50 – 12:10
Belinda Akpa, North Carolina State University, USA*
Multiscale Modeling of Plant Vacuole Fusion in Guard Cells: Positioning HOPS as the Key Regulator of Stoma Morphology

12:10 – 13:10

Session III: Fusion Pore I
Jakob B. Sørensen, University of Copenhagen, Denmark, Chair

13:10 – 13:40
Patrik Rorsman, OCDEM, United Kingdom
Insulin Exocytosis: Normal Physiology and Disruption in Type-2 Diabetes

13:40 – 14:10
Uri Ashery, Tel-Aviv University, Ramat Aviv, Israel
Fusion Pore Constriction Controls the Dynamics of Vesicular Content Release

14:10 – 14:30
Agata Witkowska, Max Planck Institute for Biophysical Chemistry, Germany*
Insights on SNARE-Mediated Fusion Learned From Early Membrane Fusion Intermediates

14:30 – 16:00
Break/Poster Session I

Session IV: Curvature
Jenny Hinshaw, NIH, USA, Chair

16:00 – 16:30
Michelle Knowles, University of Denver, USA
Protein Regulation of Exosome Secretion

16:30 – 17:00
Ravi Radhakrishnan, University of Pennsylvania, USA
Biophysics of Membrane Curvature Remodeling at Molecular and Mesoscopic Length Scales

17:00 – 17:30
Mohsen Sadeghi, Freie Universität Berlin, Germany
Parametric Particle-Based Model for Large-Scale Simulations of Membrane Dynamics

17:30 – 17:50
Comert Kural, The Ohio State University, USA*
Curvature Generation by Endocytic Clathrin Coats

18:00 – 19:30
Tour of the Botanical Garden

Dinner on own

Wednesday, May 8, 2019

8:45 – 11:00
Tour of the Historical University

Lunch on own

12:30 – 19:00

Session V: Fission
Jenny Hinshaw, NIH, USA, Chair

13:00 – 13:30
Thomas Pucadyil, Indian Institute of Science Education and Research, India
ATP-Dependent Membrane Remodeling Links EHD1 Functions to Endocytic Recycling

13:30 – 14:00
Patricia Bassereau, Institut Curie, France
ESCRT-III Filaments Have Opposite Curvature-Related Orientations on Membranes

14:00 – 14:30
Jeanne Stachowiak, University of Texas at Austin, USA
Intrinsically Disordered Proteins as Physical Drivers of Membrane Fission

14:30 – 14:50
Henry Nguyen, University of California, San Francisco, USA*
Cryo-EM Structures Reveal Progressive Membrane Constriction by the ESCRT-III Proteins IST1 and CHMP1B

14:50 – 16:20
Break/Poster Session II

Session VI: Synapses
Morten Gram Pedersen, University of Padova, Italy, Chair

16:30 – 17:00
Takeshi Sakaba, Doshisha University, Japan
Comparison of the Transmitter Release Properties Between the Calyx of Held Synapse and Mossy Fiber-CA3 Synapse

17:00 – 17:30
Alexander Walter, Leibniz-Institut für Molekulare Pharmkologie (FMP), Germany
Release Site Recruitment and Activation as Mechanisms of Presynaptic Plasticity

17:30 – 18:00
Stephanie Gupton, University of North Carolina at Chapel Hill, USA
An Unbiased Classification Approach Reveals Multiple Fusion Categories of VAMP2-Mediated Exocytosis

18:00 – 18:15

Session VII: Superresolution
Dinah Loerke, University of Denver, USA, Chair

18:15 – 18:45
Susan Cox, King's College London, United Kingdom
Faster and Better: Taking Localization Microscopy Into Live Cells

18:45 – 19:15
Katharina Gaus, University of New South Wales, Australia
Single Molecule Localization Microscopy of Receptor Signaling

Dinner on own


Thursday, May 9, 2019

Session VII: Fusion Pore II
Sebastian Barg, Uppsala University, Sweden, Chair

8:00 – 19:00

8:30 – 9:00
Ling-Gang Wu, NINDS, NIH, USA
Visualizing Membrane Structural Remodeling During Fusion and Fission in Live Cells

9:00 – 9:30
Manfred Lindau, Cornell University, USA
The Structure of the Fusion Pore

9:30 – 9:50
Raya Sorkin, Vrije Univit Amsterdam, Netherlands*
Membrane Binding, Bending, and Remodeling by Synaptotagmin-1 and Doc2b

9:50 – 10:10
Rafael Lira, Max Planck Institute, Germany*
How and How Much Has it Fused? Detecting Fusion Intermediates and Quantifying Fusion Efficiency

10:10 – 10:40
Coffee Break

Session IX: Exocytosis II
Jakob B. Sørensen, University of Copenhagen, Denmark, Chair

10:40 – 11:10
Frederic Meunier, Queensland Brain Institute, Australia
Need for Speed: Dynamic Nanoclustering and Unclustering of Munc18/Syntaxin-1 During Exocytosis

11:10 – 11:40
Giuliana Cortese, University of Padova, Italy
Advanced Statistics for Spatio-Temporal Events of Exocytosis

11:40 – 12:00
Nikhil Gandasi, Uppsala University, Sweden*
Birth of a Nanodomain: Vesicle Docking is Initiated by Rab3 Positive Vesicles Identifying Rim Sties to Tether at the Plasma Membrane

12:00 – 13:00

Session X: Fusion Proteins
Morten Gram Pedersen, University of Padova, Italy, Chair

13:00 – 13:30
Herre J Risselada, University of Göttingen, Germany
Molecular Simulations of Membrane Fusion and Related Events

13:30 – 13:50
Pavel Jungwirth, IOCB Prague, Czech Academy of Sciences, Czech Republic*
Cell Penetration and Membrane Fusion: Two Sides of the Same Coin

13:50 – 14:10
Mahmoud Moradi, University of Arkansas, USA*
Influenza Hemagglutinin-Mediated Membrane Fusion: An All-Atom Molecular Dynamics Study

14:10 – 14:30
James Munro, Tufts Unversity, USA*
Conformational Dynamics Related to Membrane Fusion Observed in Single Viral Envelope Glycoproteins

14:30 – 16:00
Break/Poster Session III

Session XI: Neuronal Endocytosis
Sebastian Barg, Uppsala University, Chair

16:00 – 16:30
Jürgen Klingauf, University of Münster, Germany
Visualizing Compensatory Endocytosis Dynamics in 'Xenapses,' TIRFM-Amendable Synapses

16:30 – 17:00
Ira Milosevic, European Neuroscience Institute Göttingen, Germany
Novel Functions of Endophilins-A in Exocytosis and Membrane Trafficking

17:00 – 17:15

Keynote Lecture
Jakob B. Sørensen, University of Copenhagen, Denmark, Chair

17:15 – 18:00
Axel Brunger, Stanford University, USA
Molecular Mechanisms of Synaptic Neuronal Exocytosis



Friday, May 10, 2019

8:00 – 12:30

Session XII: Mitochondrial Fusion and Fission
Jenny Hinshaw, NIH, USA, Chair

8:30 – 9:00
Karin Busch, University of Münster, Germany
Mitochondrial Membrane Dynamics Versus Steady Compartmentalization: A Contradiction? What Superresolution Imaging Can Tell Us

9:00 – 9:30
Luca Scorrano, University of Padova, Italy
Consequences of Mitochondrial Fusion Changes

9:30 – 9:50
Dora Mahecic, EPFL Institute of Physics, Switzerland*
Membrane Bending Energy and Tension Govern Mitochondrial Division

9:50 – 10:10
Katja Faelber, Max Delbrück Center, Germany*
Structural Insights Into Mitochondrial Inner Membrane Remodeling

10:10 – 10:40
Coffee Break

Session XIII: Exocytosis III
Jakob B. Sørensen, University of Copenhagen, Denmark, Chair

10:40– 11:10
Fernando Marengo, University of Buenos Aires, Argentina
Endocytosis and Vesicle Replenishment After the Exocytosis of the Immediately Releasable Pool in Mouse Chromaffin Cells

11:10 – 11:40
Aleksandra Radenovic, École Polytechnique Fédérale de Lausanne, Switzerland
The Power of Correlative Superresolution Imaging

11:40 – 12:00
Francesco Montefusco, University of Padova, Italy*
How is Granule Release Affected by Location and Number of Different Types of Ca2+ Channels? Markov Chain Models Provide Analytic Results

12:00 – 12:15
Closing Remarks and Biophysical Journal Poster Awards Presentation
Morten Gram Pedersen, University of Italy, Padova, Italy

Lunch on own

*Short talks selected from among submitted abstracts