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Daily Schedule



Saturday, June 17, 2017

8:00 AM - 6:00 PM
Registration/ Information

8:55 AM – 9:00 AM
Opening Remarks
Jung-Chi Liao, Institute of Atomic and Molecular Sciences, Academia Sinica, Taiwan

Session I: Advanced Microscopy for Single Cell Studies
Jung-Chi Liao, Institute of Atomic and Molecular Sciences, Academia Sinica, Taiwan, Chair

9:00 AM - 9:25 AM
Suliana Manley, École Polytechnique Fédérale de Lausanne, Switzerland
The Tortoise and the Hare: Bacteria and Mitochondria Division Dynamics Revealed by Time-lapse Superresolution Microscopy

9:25 AM - 9:50 AM
Bi-chang Chen, Academia Sinica, Taiwan
Lattice Light Sheet Microscopy on Single-Cell Imaging

9:50 AM - 10:15 AM
Shean-jen Chen, National Chiao Tung University, Taiwan
Deep-Biotissue Imaging by Temporal Focusing Widefield Multiphoton Microscopy

10:15 AM - 10:35 AM
Masahiro Ueda, Osaka University, Japan *
Automated Imaging System for Single-molecule Analysis in Living Cells

10:35 AM - 11:05 AM
Coffee Break

Session II: Single Cell Mechanobiology I
Yujie Sun, Biodynamics Optical Imaging Center, Peking University, China, Chair

11:05 AM - 11:30 AM
Taekjip Ha, Johns Hopkins University, USA
Playing a Tug of War with Membrane Receptors Using the Double Helix

11:30 AM - 11:55 AM
Pakorn Kanchanawong, National University of Singapore, Singapore
Nanoscale Architecture of Cadherin-based Cell Adhesions

11:55 AM - 12:15 PM
Ashley Nord, Centre de Biochimie Structurale, France*
Stator Stoichiometry and Mechanosensitivity of the Bacterial Flagellar Motor Probed by Load Manipulation

12:15 PM - 1:30 PM
Lunch

1:30 PM - 3:00 PM
Poster Session I

Session III: Nanotechnology in Single Cell Biology
Takeharu Nagai, Osaka University, Japan, Chair

3:00 PM - 3:25 PM
Bianxiao Cui, Stanford University, USA
Membrane Curvature at the Nano-bio Interface

3:25 PM - 3:50 PM
Haw Yang, Princeton University, USA
The Multi-resolution Imaging Approach to Nano-Bio Interactions at the Single-Cell Level

3:50 PM - 4:10 PM
Scott Thourson, Georgia Tech, USA *
Localized Modulation of Single Cardiomyocytes Using PEDOT:PSS Conducting Polymer Microwires

4:10 PM - 4:40 PM
Coffee Break

Session IV: Cellular Processes in Single Cells I: Cell Cycle
Jie Xiao, Johns Hopkins University, USA, Chair

4:40 PM - 5:05 PM
Paul Wiggins, University of Washington, USA
The Replisome Undergoes Multiple Rounds of Disassembly and Restart Every Cell Cycle

5:05 PM - 5:30 PM
Sheng-hong Chen, Institute of Atomic and Molecular Sciences, Academia Sinica, Taiwan
Protein Dynamics in Single Cells Unveil Regulatory and Therapeutic Principles

5:30 PM - 5:50 PM
Yuan Lin, University of Hong Kong *
Shape Transformation of the Nuclear Envelope During Closed Mitosis

6:00 PM - 7:30 PM
Opening Reception

7:30 PM
Night Market Tour

Sunday, June 18, 2017

8:30 AM - 1:30 PM
Registration/Information

Session V: Cellular Processes in Single Cells II: Transcription
Sua Myong, Johns Hopkins University, USA, Chair

9:00 AM - 9:25 AM
Achillefs Kapanidis, Oxford University, United Kingdom
Bacterial Transcription Meets Chromosome Organisation: a Single-molecule Perspective

9:25 AM - 9:50 AM
Nam Ki Lee, Pohang University of Science and Technology, South Korea
Direct Observation of Transcription in a Living Bacterial Cell

9:50 AM - 10:15 AM
David Rueda, Imperial College London, United Kingdom
Imaging Small Cellular RNAs with Fluorescent Mango RNA Aptamers

10:15 AM - 10:35 AM
Xiaoli Weng, Johns Hopkins University, USA*
Spatial Organization of Transcription in E. coli via Superresolution Fluorescence Microscopy

10:35 AM - 11:05 AM
Coffee Break

Session VI: Single Cell Modeling
Wallace Marshall, University of California, San Francisco, USA, Chair

11:05 AM - 11:30 AM
Phillip Nelson, University of Pennsylvania, USA
Old and New News about Single-Photon Sensitivity in Human Vision

11:30 AM -  11:50 AM
Po-Yi Ho, Harvard University, USA*
Interrogating the Bacterial Cell Cycle by Cell Dimension Perturbations and Stochastic Modeling

11:50 AM - 12:10 PM
Rosanna Smith, University of Southampton, United Kingdom*
The Observer Effect in Cell Biology: Gene Expression Noise, Genetic Reporters and the Problem of Measurement in Live Cells

12:10 PM - 1:30 PM
Lunch

1:30 PM
Culture Tour, Dinner on own

Monday, June 19, 2017

8:30 AM - 6:00 PM
Registration/Information

Session VII: Cellular Processes in Single Cells III: Cell Shape and Size
Paul Wiggins, University of Washington, USA, Chair

9:00 AM - 9:25 AM
Wallace Marshall, University of California, San Francisco, USA
Intrinsic and Extrinsic Noise in an Organelle Size Control System

9:25 AM - 9:50 AM
KC Huang, Stanford University, USA
Cell-size Determination by the Bacterial Actin Cytoskeleton

9:50 AM - 10:15 AM
Chien-jung Lo, National Central University, Taiwan
How Fast Can Bacteria Grow Their Flagella?

10:15 AM - 10:35 AM
Tony Yang, Academia Sinica, Taiwan*
Intraflagellar Transport Proteins Undergo Nonaxonemal Staged Hindrance Between the Recruiting Distal Appendages and the Cilium

10:35 AM - 11:05 AM
Coffee Break

Session VIII: Single Cell Sequencing
Achillefs Kapanidis, Oxford University, United Kingdom, Chair

11:05 AM - 11:30 AM
Sunney Xie, Harvard University, USA; Peking University, Beijing Advanced Innovation Center for Genomics, China
Chromosomes as Single Molecules

11:30 AM - 11:55 AM
Yanyi Huang, Biodynamics Optical Imaging Center, Peking University, China
Microfluidic Single Cell Sequencing

11:55 AM - 12:15 PM
Jung-Ming Lin, University of California, Berkeley, USA*
Single-Cell Proteotypic Analysis of Invasive Motility in Glioblastoma

12:15 PM - 1:30 PM
Lunch

1:30 PM - 3:00 PM
Poster Session II

Session IX: Cellular Processes in Single Cells IV: Chromosome Dynamics
Christine Payne, Georgia Institute of Technology, USA Chair

3:00 PM - 3:25 PM
Johan Elf, Uppsala University, Sweden
Single Molecule Studies of Cas9 Search Kinetics in Living Cells

3:25 PM - 3:50 PM
Melike Lakadamyali, Institute of Photonic Sciences, Spain
Decoding Chromatin Organization with Superresolution Microscopy

3:50 PM - 4:15 PM
Yujie Sun, Biodynamics Optical Imaging Center, Peking University, China
Single Molecule Study of the Chromatin Structure and Dynamics

4:15 PM - 4:35 PM
Sangyoon Han, University of Texas Southwestern Medical Center, USA*
Emerging Role of Differential Molecular Association in Force-transmitting Nascent Adhesions

4:35 PM - 5:05 PM
Coffee Break

Session X: Single Cell Mechanobiology II
Haw Yang, Princeton University, USA, Chair

5:05 PM - 5:30 PM
Megan Valentine, University of California, Santa Barbara, USA
Disruption of Cellular Force-sensing Triggers Systemic Tissue Collapse in the Botryllus Vasculature

5:30 PM - 5:55 PM
Chin-lin Guo, Institute of Physics, Academia Sinica, Taiwan
Spontaneous Patterning of Cytoskeleton in Single Epithelial Cell Apicobasal Polarity Formation

5:55 PM - 6:15 PM
Poul Bendix, Niels Bohr Institute, Denmark*
Rotation, Twisting and Pulling: the Rich Dynamics of Filopodia

7:30 PM
Banquet

Tuesday, June 20, 2017

8:30 AM - 12:00 PM
Registration/Information

Session XI: Sensor and Probe Development
David Rueda, Imperial College London, United Kingdom, Chair

9:00 AM - 9:25 AM
Amy Palmer, University of Colorado Boulder, USA
Quantitative Biology with Genetically Encoded Sensors – Opportunities and Challenges

9:25 AM - 9:50 AM
Takeharu Nagai, Osaka University, Japan
Acid Resistant Monomeric GFP for Quantitative Single Cell Analyses

9:50 AM - 10:15 AM
Sua Myong, Johns Hopkins University, USA
Single mRNA Counting in Single Cell Reveals Function of RNA Stem Structure in Coupling Dicing and Gene Silencing

10:15 AM - 10:35 AM
Ralph Jimenez, University of Colorado, Boulder, USA*
Systematic Evaluation of Cellular Zn2+ Sensors with Microfluidic Cytometry

10:35 AM - 11:05 AM
Coffee Break

Session XII: Cellular Processes in Single Cells V: DNA Replication and Repair
Keng-Hui Lin, Institute of Physics, Academia Sinica, Taiwan, Chair

11:05 AM - 11:30 AM
Julie Biteen, University of Michigan, USA
Single-molecule Investigations of DNA Replication and Repair in Living Bacteria

11:30 AM - 11:55 AM
Antoine van Oijen, University of Wollongong, Australia
Single-molecule Visualization of Bacterial DNA Repair in Live Cells

11:55 AM - 12:15 PM
Nicholas Kurniawan, Eindhoven University of Technology, Netherlands*
Local 3D Single-Cell–Matrix Interactions Underlie the Spatiotemporal Dynamics of Cell Populations

12:15 PM - 12:20 PM
Closing Remarks and Biophysical Journal Poster Awards
Keng-Hui Lin, Institute of Physics, Academia Sinica, Taiwan

*Contributed talks selected from among submitted abstracts