Symposia

Sunday, March 6

11:00 AM - 12:30 PM

Recent Advances in High Resolution Imaging and Nanomedical Measurements Using AFM
 
Bruker Corp.

1:00 PM - 2:30 PM

Automated Patch Clamp Workshop - Live Experiments
 
Nanion Technologies GMGH

3:00 PM - 4:30 PM

Probing the Nanomechanics of Biological Systems with an AFM - Instrumentation and Applications
 
Asylum Research
 
Atomic Force Microscopy (AFM) offers a reliable approach to measuring the nanomechanical properties of biological systems. During this tutorial we will focus predominately on elasticity measurements of cells which are crucial to understanding basic cellular function (motility, adhesion, differentiation, apoptosis), cytoskeletal dynamics and disease. We will discuss instrumentation including optical integration with the AFM, data acquisition/force mapping and data processing. Several examples will be presented.

5:00 PM - 6:30 PM

Coupling Super-Resolution with Electron Microscopy, Dr. Erik M. Jorgensen, Ph.D., HHMI 

Vari Group

 

Monday, March 7

9:00 AM - 10:30 AM

FluidFM: Combining Atomic Force Microscopy and Nanofluidics in a Universal Liquid Delivery System for Single Cell Applications and Beyond
 
Nanosurf, Inc.
 
The fluidFM is a new technology based on Nanosurf's FlexAFM platform combined with cantilever technology developed by Cytosurge. The atomic force microscope (AFM) utilizes hollow cantilevers for local liquid dispensing and stimulation of single living cells under physiological conditions. A nanofluidic channel in the cantilever allows soluble molecules to be dispensed through a submicrometer aperture in the AFM tip. The sensitive AFM force feedback allows controlled approach of the tip to a sample for extremely local modification of surfaces in liquid environments. It also allows reliable discrimination between gentle contact with a cell membrane or its perforation. Using these two procedures, dyes have been introduced into individual living cells and even selected subcellular structures of these cells. The universality and versatility of the fluidFM will stimulate original experiments at the submicrometer scale not only in biology but also in physics, chemistry, and material science.

11:00 AM - 12:30 PM

IonFlux System:  Automated Patch Clamp with Plate Reader Simplicity
 
Fluxion Biosciences

1:00 PM - 2:30 PM 

A Next Generation Bio-AFM
 
Nanonics Imaging LTD
www.nanonics.co.il
 
New levels of ultrasensitivity in scanned probe bioimaging and sensing will be described.  The advances derive from a new feedback mechanism for AFM imaging in liquids that has been introduced recently.  Over the last decade it has been increasingly recognized that tuning fork technology is the most effective method for AFM feedback.  This is a result of ultrasharp resonances with high Q factors with no jump to contact.  However, the clear advantages of tuning forks were prevented from advancing BioAFM since tuning forks did not work in biological media.  This has been overcome by a singular advance that has permitted tuning fork probes to work in biological media and thus, to be extended into biological functional imaging and sensing of living systems.  This is achieved with no need for artificial corrections for Q factor as is the case in beam bounce AFM technology with standard silicon cantilevers.  Thus, the ultrasensitivity and high resolution achievable with tuning fork based probes can now be fully realized for BioAFM imaging.  An additional very important advantage is that such tuning fork feedback provides no optical interference and a platform will be introduced that can be fully integrated with any upright, inverted or 4 pi microscope.  This significantly simplifies integrations of optical methods with AFM such as fluorescence, Raman, TERS, TIRF and FRET.  It also readily permits integration with all non-linear optical techniques even those such as second harmonic generation where illumination and collection is best implemented on opposite sides of the sample.  In addition as part of this advance a NanoToolKitTM of tuning fork probes will be presented that allow ultrasensitive force measurements including protein pulling, elasticity, adhesion, thermal and electrical probing, ion conductance etc.  Tuning forks allow all probes with a variety of force constants even those above 10 N/m such as NSOM and electrochemical probes to be employed on living cells.  All such probes have non-obscuring cantilevers with highly extended probe tip design and not even  DIC imaging is affected by their presence.  These geometries also allow for the unique advantages of multiprobe AFM to be extended to BioAFM.  Thus, AFM can now be combined on-line with a variety of other probe functionalities including on-line normal force near-field optical super-resolution & FCS, electrochemical imaging, ion conductance sensing etc. 

3:00 PM - 4:30 PM

SensiQ Pioneer - Features & Benefits
 
ICX Nomadics

5:00 PM - 6:30 PM

Getting the Most Out of pCLAMP(r) Software
 
Molecular Devices, Inc.
 
The Axon™ User Meeting provides users of Axon microelectrode amplifiers, digitizers, and electrophysiology data acquisition and analysis software an opportunity to hear about the latest developments in electrophysiology. There will be presentations by scientists demonstrating how Molecular Devices Conventional Electrophysiology products are used efficiently in scientific research. In addition, there will be time to network with colleagues and share experiences and solutions to common problems. Experts from Molecular Devices will be available to provide answers to your technical and scientific questions and to collect users' input on current and future products.
 

Tuesday, March 8

11:00 AM - 12:30 PM

Advances in fluorescence based water contaminants analysis
 
HORIBA Scientific

 

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