Sunday,  September 24, 2023

5:00 PM - 8:45 PM

7:00 PM - 7:10 PM
Welcome and Opening Remarks
Ling-Gang Wu, NIH, USA

Session I: Keynotes I
                  Ling-Gang Wu, NIH, NINDS, USA, Chair

7:10 PM - 7:35 PM
John Heuser, Washington University in St. Louis, USA
Revisiting Electron Microscopy's "Golden Decade" of Imaging Synaptic Vesicle Dynamics: The 1970's

7:35 PM - 8:00 PM
Edwin Chapman, University of Wisconsin-Madison, USA
Unexpected Interplay Between Two Presynaptic Calcium Sensors

8:00 PM - 8:45 PM
James Rothman, Yale University, USA
Turbo-Charging Synaptic Vesicles for Explosive Release of Neurotransmitters

Monday, September 25, 2023

7:30 AM - 8:30 AM

8:00 AM - 5:00 PM

Session II: Dense Core Vesicle Fusion and Fission
                   Volker Kiessling, University of Virginia, USA, Chair

8:30 AM - 8:55 AM
Erdem Karatekin, Yale University, USA
Membrane Fusion and Fission: Lessons from Unexpected Places

8:55  AM - 9:10 AM
Kobina Essandoh, University of Michigan, USA*
Molecular Regulation of Atrial Natriueretic Peptide Secretion in Cardiomyocytes by the RAB3GAP1 and GTPASE Cycle

9:10 AM - 9:35 AM
Arun Anantharam, The University of Toledo, USA
Signaling Networks Coupling Calcium to Secretion in the Adrenal Medulla

9:35 AM - 10:00 AM
Ira Milosevic, Oxford University, United Kingdom
Negative Regulation of Exocytosis by Amisyn

10:00 AM - 10:30 AM

Session III: Intra/Inter-Cellular Fusion and Fission
                    TBD, Chair

10:30 AM - 10:55 AM
Aurelian Roux, University of Geneva, Switzerland
Reconstituting ESCRT-III Mediated Membrane Fission In Vitro

10:55 AM - 11:20 AM
Sandy Maday, University of Pennsylvania, USA
Autophagy-Lyosomal Pathways in Neurons and Astrocytes

11:20 AM - 11:45 AM
Michael Kozlov, Tel Aviv University, Israel
Model for Ring Collapse and Membrane Fission in ER Tubular Networks

11:45 AM - 12:00 PM
Lisa Redlingshofer, Max Planck Institute, Germany*
Reconstructing the Collective Behaviour of the Endosomal Membrane Fusion  Machinery

12:00 PM - 12:25 PM
Elizabeth Chen, University of Texas, Southwestern Medical Center, USA
Function of ABC G1/G4 Transporters and Cholesterol in Cell-Cell Fusion

12:25 PM - 1:25 PM

Session IV: Endocytosis, Membrane Bending and Fission
                    Sarah Nyenhuis, NIH, USA, Chair

1:35 PM - 2:00 PM
Thomas Pucadyil, Indian Institute of Science and Education and Research, India
Mechanistic Analysis of Membrane Fission and Discovery of Novel Fission Proteins

2:00 PM - 2:25 PM
David Drubin, University of California, Berkeley, USA
Harnessing Actin-Assembly Forces to Drive Vesicle Formations During Clathrin-Mediated Endocytosis

2:25 PM - 2:40 PM
Richard Hooy, University of California, Berkeley, USA*
Self-Assembly and Structure of a Clathrin-Independent AP-1: ARF1 Tubular Membrane Cell

2:40 PM - 3:05 PM
Vadim Frolov, Ikerbasque-Basque Foundation for Science, Spain
Molecular Mechanosensing  in Dynamin-Driven Membrane Remodeling

3:15 PM - 4:45 PM
Poster Session I

4:45 PM - 7:30 PM
Free Time/Dinner on Own

Session V:  Extracellular and Mitochondrial Vesicles
                     Prabhodh Abbineni, Loyola University, Chicago, USA, Chair

Chantell Evans, Duke University, USA
Mitochondrial Derived Vesicles: A New Mitochondrial Quality Control Mechanism

7:55 PM - 8:20 PM
Alissa Weaver, Vanderbilt University, USA
Biogenesis of RNA-Containing Extracellular Vesicles at ER-Membrane Contact Sites

8:20 PM - 8:45 PM
Ann Wehman, University of Denver, USA
Extracellular Vesicles Budding and Lipid Asymmetry

8:45 PM - 9:10 PM
Michelle Knowles, University of Denver, USA
Multivesicular Endosome Fusion Kinetics Depend on Exosome Tethering and Temperature

Tuesday, September 26, 2023

7:30 AM - 8:30 AM

8:30 AM - 4:15 PM

Session VI: Synaptic Vesicle Fusion
                     Jianyuan Sun, Chinese Academy of Sciences, China, Chair

8:30 AM - 8:55 AM
Ege Kavalali, Vanderbilt University, USA
Functional Nano-Organization of Synapses

8:55 AM - 9:10 AM
Amelia Ralowicz, Dartmouth College, USA*
Spontaneous Synaptic Vesicles Function is Correlated with Number of Vesicles in Readily Releasable Pool

9:10 AM - 9:35 AM
Jeremy Dittman, Cornell University, Weill Medical College, USA
Molecular Insights into Mun13 and Complexin Function at the Synapse

9:35 AM - 10:00 AM
Skyler Jackman, Oregon Health and Science University, USA
High-Affinity Calcium Sensors Mediate Asynchronous Vesicle Fusion

10:00 AM - 10:30 AM
Coffee Break

Session VII: Fusion and Fission in Infection and Disease
                      Katya Heldwein, Tufts University, USA, Chair

10:30 AM - 10:55 AM
Carol Parent, University of Michigan, USA
The Neutrophil Nucleus as a Signaling Hub during Neutrophil Chemotaxis

10:55 AM - 11:20 AM
Gregory Melikian, Emory University, USA
HIV-1 Preferentially Fuses with PH-Neutral Vesicles in Cell Lines and Primary CD4+ T-Cells

11:20 AM - 11:35 AM
Xin Wang*
Macropinocytosis in Sars CoV2 Infection

11:35 AM - 11:50 AM
Gonzalo Gonzalez-Del Pino, Tufts University, USA*
Structural and Functional Characterization of the Herpes Simplex Virus Membrane Fusion Regulator, GH/GL

11:50 AM - 12:15 PM
Comert Kural, Ohio State University, USA*
Sensitization of Cancer Cells to FAS-Induced Apoptosis Through Endocytosis Inhibition

12:15 PM - 1:15 PM

Session VIII: Membrane Bending, Fusion, and Endocytosis
                      Ioana Butu, Columbia University, USA, Chair

1:25 PM - 1:50 PM
Wenting Zhao, Nanyang Technological University, Singapore
Nanoscale Membrane Topography Engineering: In Cells, Invitro and In Between

1:50 PM - 2:15 PM
Ben O'Shaugnessy, Columbia University, USA
Mechanical Regulation of Membrane Fusion, Exocytosis and Endocytosis Studied by Coarse-Grained
Simulation and Experimental Data Analysis

2:15 PM - 2:30 PM
Aline Tschanz, EMBL, Germany*
Clathrin Coats Partially Preassemble and Subsequently Bend During Endocytosis

2:30 PM - 2:55 PM
Marko Kaksonen, University of Geneva, Switzerland
Evolutionary Cell Biology Approach to Clathrin-Mediated Endocytosis

3:05 PM - 4:35 PM
Poster Session II

4:35 PM - 7:30 PM
Free Time/Dinner on Own

Session IV: Dense Core Vesicle Fusion and Endocytosis
                    Debasis Das, Tata Institute of Fundamental Research, India, Chair

7:30 PM - 7:55 PM
Ling-Gang Wu, NIH, NINDS, USA
Dynamics of Membrane Fusion and Endocytosis in Secretory Cells

7:55 PM - 8:20PM
Lukas Tamm, University of Virginia, USA
Membrane Lipids Couple Synaptotagmin to SNARE Mediated Granule Fusion in Insulin Secreting Cells

8:20 PM - 8:45 PM
Manfred Lindau, University of Miami, USA
All SNAP25 Copies in the Vesicle-Plasma Membrane Contact Zone Change Conformation During Vesicle Priming 

8:.45 PM - 9:10 PM
Corey Smith, Case Western Reserve University, USA
Rapid Electrochemical Measure of Neurotransmitter Dynamics in a Beating Pig Heart

Wednesday, September 27, 2023

7:00 AM - 8:30 AM

8:30 AM - 4:30 PM

Session X: Endocytosis and Membrane Bending
                   Jefferson Knight, University of Colorado, USA

8:30 AM - 8:45 AM
Santiago Di Pietro, Colorado State University, USA*
Mechanism of Actin Capping Protein Recruitment and turnover During Clathrin Mediated Endocytosis

8:45 AM - 9:10 AM
Justin Taraska, NIH, USA
Measuring the Nanoscale Structure of Endocytosis with Light and Electron Microscopy

9:10 AM - 9:35 AM
Margaret Johnson, Johns Hopkins University, USA
Membrane Bending and Assembly Costs for Clathrin Coat Formation

9:35 AM - 10:00 AM
Shigeki Watanabe, Johns Hopkins University, USA
Dynamin Prime- Accelerating Endocytosis at Synapses

10:00 AM - 10:30 AM
Coffee Break

Session XI: Synaptic Vesicle Fusion and Endocytosis
                     Chair: Jiajie Diao, University of Cincinnati, USA

10:30 AM - 10:55 AM
Noa Lipstein-Thoms, Max Planck Institute for Multidisciplinary Sciences, Germany
Human UNC13A Gene Variations Cause a Neurodevelopmental Syndrome and Impair Synaptic Function

10:55 AM - 11:20 AM
Stephanie Gupton, University of North Carolina Chapel Hill, USA
Spatial and Temporal Regulation of Constitutive Exocytosis in Cell Shape Change

11:20 AM - 11:45 AM
Michael Cousin, University of Edinburgh, United Kingdom
Dynamin-1 Dysfunction in Human Disease

11:45 AM - 12:00 PM
Stephane Gasman, Institut des Neurosciences Cellulaire et Integratives, CNRS, France*
Transbilayer Phospholipid Scrambling is Essential for Efficient Synaptic Vesicle Endocytosis

12:00 PM - 1:00 PM

Session XII: Fusion Pore and Release Mechanisms
                    Ronald Holz, University of Michigan, USA

1:10 PM - 1:35 PM
Zhuan Zhou, Peking University, China
Differential Co-Release of Catecholamine and ATP from a Vesicle Fusion Pore in Mouse Adrenal Chromaffin Cells

1:35 PM - 2:00 PM
Joshua Zimmerberg, NIH, USA
Aggregation-Dependent Lipid/Fusion Peptides Pore Formation, and Microvesicles that Secrete Exosomes

2:00 PM - 2:25 PM
Meyer Jackson, University of Wisconsin-Madison, USA
Fusion Pore Dynamics in Endocrine and Synaptic Exocytosis

2:25 PM - 2:50 PM
Sebastian Barg, Uppsala University, Sweden
Fusion Pore Regulation in Insulin-Secreting Beta Cells

3:00 PM - 4:30 PM
Poster Session III

4:30 PM - 5:15 PM
Free Time

Session XIII: Keynotes II
               Michelle Knowles, University of Denver, USA

5:15 PM - 5:40 PM
Phyllis Hanson, University of Michigan, USA
Beyond Repair: ESCRT's Enhance Membrane Resilience

5:40 PM - 6:05 PM
Jenny Hinshaw, NIH, USA
Cryo-EM Structures of Dynamin Proteins Involved in Membrane Fission and Fusion

6:05 PM - 6:50 PM
Axel Brunger, Stanford University, USA
Molecular Mechanisms of Calcium-Triggered Exocytosis

6:50 PM - 7:00 PM
Closing Remarks and Biophysical Journal Poster Awards

7:30 PM


* Contributed talks selected from among submitted abstracts