Daily Schedule

Sunday, October 14, 2012  

2:00 PM - 5:00 PM

6:00 PM - 7:00 PM
Welcome Reception

7:00 PM - 9:00 PM
Organizer/Speaker Dinner

Monday, October 15, 2012

7:00 AM - 5:00 PM

8:00 AM - 5:00 PM

7:45 AM - 8:00 AM
Opening Remarks
Zihe Rao, Nankai University, China

Session 1: NMR Spectroscopy
Chair: Angela Gronenborn, University of Pittsburgh, USA

8:00 AM – 8:25 AM
Gerhard Wagner, Harvard University, USA
Weak Interactions in the Regulation and Inhibition of Eukaryotic Translation Initiation

8:25 AM – 8:50 AM
Mingjie Zhang, Hong Kong University of Science and Technology, China
Assembling Highly Specific Signaling Complexes by Multiple Weak but Coincident Interactions 

8:50 AM – 9:15 AM
Angela Gronenborn, University of Pittsburgh, USA
Mannose-binding Lectins - Cyanovirin and Beyond 

9:15 AM – 9:40 AM
Yunyu Shi, University of Science and Technology of China, China
Cooperation of Escherichia coli Hfq Hexamers in DsrA Binding and ATP Hydrolysis

9:40 AM – 9:55 AM
Leo Spyracopoulos, University of Alberta, Canada
Accurate Thermodynamics and Kinetics for Weak Protein-Ligand Interactions Determined from NMR-monitored Chemical Shift Titrations

9:55 AM – 10:10 AM
Chun Tang, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, China
Direct Visualization of Protein Dynamics Using PRE NMR

10:10 AM – 10:50 AM
Group Photo and Break

Session 2: X-ray Crystallography
Chair: Ruiming Xu, Institute of Biophysics, Chinese Academy of Sciences, China

10:50 AM – 11:15 AM
Roy A. Mariuzza, University of Maryland, USA
Use of Directed Evolution to Stabilize Weak Protein-Protein Complexes for X-ray Crystallography 

11:15 AM – 11:40 AM
Ruiming Xu, Institute of Biophysics, Chinese Academy of Sciences, China
Structural Basis for Chaperone-Histone Interactions

11:40 AM – 11:55 AM
Yuxin Mao, Cornell University, USA
Structural Basis for Substrate Recognition by a Novel Legionella Phosphoinositide Phosphatase

11:55 AM – 1:00 PM

Session 3: Label-free Techniques
Chair: Quan Cheng, University of California, Riverside, USA

1:00 PM – 1:25 PM
Ronan O’Brien, GE Healthcare, USA
Technical Advances, Limitations, and Advantages of ITC and SPR in Detecting Protein-Ligand Interactions

1:25 PM – 1:40 PM
Quan Cheng, University of California, Riverside, USA
Probing Carbohydrate-Protein Interactions by SPR with Biotinylated Interface

1:40 PM – 1:55 PM
Michael Bova, Elan Pharmaceutical, South San Francisco, USA
Characterization of Weak Protein-Ligand Interactions Using Label Free Techniques to Support Drug Discovery

1:55 PM – 2:10 PM
Tim Cloutier, PerkinElmer Inc., U.K.
Cell and Biochemical Assay using Epic® Label-free Technology on the EnSpire® Multimode Plate Reader

2:10 PM – 2:25 PM
Darick Dayne, ForteBio - A Division of Pall Life Sciences, USA
Bio-Layer Interferometry (BLI): Instant Dip and ReadTM Label-Free Protein Quantitation and Binding Kinetics

2:25 PM – 2:40 PM
Dile Holton, TA Instrument, USA
Characterizing Weak Binding Events with Two Advanced Biophysical Techniques:  Hydrogen Deuterium Exchange (HDX-MS) and Differential Scanning Calorimetry (DSC)

2:40 PM – 3:00 PM

Session 4: SAXS and EM Approaches
Chair: Dorit Hanein, Sanford-Burnham Medical Institute, USA

3:00 PM – 3:25 PM
Dmitri Svergun, European Molecular Biology Laboratory, Germany
Structural Studies of Protein-Ligand Interactions in Solution by Small-Angle X-ray Scattering

3:25 PM – 3:50 PM
Carolyn Larabell, University of California, San Francisco, USA
Putting Molecules in Context with Correlated Cryo Confocal and X-ray Tomography

3:50 PM – 4:15 PM
Dorit Hanein, Sanford-Burnham Medical Institute, USA
Structural Mapping of the Protein Universe: One Cell at a Time

4:15 PM – 4:30 PM
Gang Cai, The University of Science and Technology, China
EM Studies of Eukaryotic Transcriptional Complexes: Structural Insights into Transcription Regulation

4:30 PM – 4:45 PM
Niels Volkmann, Sanford-Burnham Medical Research Institute, USA
Three-Dimensional Structures of Arp2/3 Complex in Actin-branch Junctions and with Bound Nucleation Promoting Factors

4:45 PM – 5:00 PM
Joseph Ferrara, Rigaku Americas Corp, USA
BioSAXS-1000: A Tool For Gaining Structural Insight When Crystallographic Methods Are Not Enough

5:15 PM – 6:45 PM
Poster Session I

7:00 PM – 9:00 PM
Dinner on own

Tuesday, October 16, 2012

Excursion to the Great Wall   

12:00 PM – 1:00 PM

1:00 PM – 5:00 PM

1:00 PM - 5:00 PM

Session 5: Single Molecule Methods
Chair: Gil Lee, University College Dublin, Ireland 

1:00 PM – 1:25 PM
Gil Lee, University College Dublin, Ireland
Magnetic Tweezers and Atomic Force Microscope Reveal Mechanically Distinct apCAM Domain Interactions

1:25 PM – 1:40 PM
Will Hancock, Pennsylvania State University, USA
Processivity Mechanisms Across Diverse Kinesin Motor Protein Families

1:40 PM – 1:55 PM
Jiajie Diao, Stanford University, USA
Single Molecule Studies of Syntaxin and Munc18 Interactions during SNARE-Mediated Membrane Fusion

1:55 PM – 2:10 PM
Baohua Ji, Beijing Institute of Technology, China
Stiffness Dependent Rupture Force of Ligand-Receptor Bond at Ultralow Loading Rate

2:10 PM – 2:30 PM

Session 6: New Methods 
Chair: Chun Tang, Chinese Academy of Sciences, China

2:30 PM – 2:45 PM
Mauricio Carrillo-Tripp, Langebio-Cinvestav, Mexico
Linking Enzyme Conformational Diversity and Substrate Specificity through Molecular Thermodynamic Analysis

2:45 PM – 3:00 PM
Jianfeng Liu, Huazhong University of Science and Technology, Wuhan, China
An Activity-based Probe Reveals Dynamic Protein-Protein Interactions Mediating IGF-1R Transactivation by the GABAB Receptor

3:00 PM – 3:15 PM
Alexander Fonin, Institute of Cytology of the RAS, Saint-Petersburg, Russian Federation
Pitfalls in the use of Fluorescence for Determination Ligand-Receptor Binding Stoichiometry and Binding Constants

3:15 PM – 3:30 PM
Changlin Tian, University of Science & Technology, Heifei, China
Chemical Shift and Relaxation Comparisons of Membrane in its Native Environment Versus Detergent Micelles Using 19F Site-specific Labeling and 19F Nuclear Magnetic Resonance

3:30 PM – 5:00 PM
Poster Session II

7:00 PM – 9:00 PM

Wednesday, October 17, 2012

7:00 AM - 5:00 PM
Information Desk

8:00 AM - 3:00 PM

Session 7: Computational Methods
Chair: Alexandre Bonvin, Utrecht University, The Netherlands

8:00 AM – 8:25 AM
Alexandre Bonvin, Utrecht University, The Netherlands
Modeling Protein-Protein Interactions by Information-driven Docking

8:25 AM – 8:40 AM
Jung-Hsin Lin, Academia Sinica, Taipei, Taiwan
Development of Scoring Functions for Protein-Ligand Interactions based on Quantum Chemical Charge Models and Robust Regression Analysis: Performance on Weakly Interacting Complexes?

8:40 AM – 8:55 AM
Art Cho, Korea University, Seoul, South Korea
Quantum Mechanical Energies in Protein-Ligand Binding, How Important Are They?

8:55 AM – 9:10 AM
Matthias Buck, Case Western Reserve University, Cleveland, USA
Protein Association with Alternatives: Investigations into Dynamic Protein Complexes by Microsecond all Atom Molecular Dynamics Simulations and Comparison with Solution NMR Measurements

9:10 AM – 9:25 AM
Mohammad R.K. Mofrad, University of California, Berkeley, USA
Integrin-Mediated Focal Adhesion Mechanotransduction: Exploring the Molecular Details of ‘Outside-In’ and ‘Inside-Out’ Signaling

9:25 AM – 9:40 AM
David Green, Stony Brook University, New York, USA
Deconstructing Carbohydrate-Lectin Interactions through Simulation

9:40 AM - 10:00 AM

Session 8: Drug Design
Chair: Hualiang Jiang, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China

10:00 AM – 10:25 AM
Hualiang Jiang, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
Constructing the Complete Free Energy Landscape of Ligand-Protein Binding

10:25 AM – 10:40 AM
Jianing Li, University of Chicago, USA
Multiscale Modeling of GPCR-Ligand Interactions for Structure-based Drug Discovery

10:40 AM – 10:55 AM
Tian Zhu, University of Illinois at Chicago, USA
Fragment-Based Drug Discovery Using a Multi-Domain, Parallel MM/PBSA Screening Protocol Against A Novel, Antibacterial Target

10:55 AM – 11:10 AM
Ke Ruan, University of Science and Technology, Hefei, China
Weak Binders to Leukemia Associated RhoGEF Probed by NMR Fragment Based Screening

11:10 AM – 11:25 AM
Shoucheng Du, University of Pittsburgh, Pittsburgh, USA
Development of HIV-1 Capsid Assembly Inhibitors, A Structure-based Conformational-Trapping Approach

11:30 AM – 1:00 PM
Break and Lunch

Session 9: Biochemical and In Vivo Methods I
Chair: Rong Li, Stowers Institute for Medical Research, USA

1:00 PM – 1:25 PM
Marie-France Carlier, National Center for Scientific Research, France
Multifunctional Regulation of Actin Assembly by WASP Homology 2 Domains

1:25 PM – 1:50 PM
Rong Li, Stowers Institute for Medical Research, USA
Mechanism of Cell Polarization in Budding Yeast

1:50 PM – 2:05 PM
Tae-Yong Yoon, KAIST, South Korea
Real-Time Single-Molecule Co-Immunoprecipitation Analyses Reveal Cancer-Specific Ras Signaling Dynamics

2:05 PM – 2:20 PM
Alla Kostyukova, Washington State University, Pullman, USA
Using Putative Binding Sites for Ligand Catching

2:20 PM – 2:45 PM
Tom Kerppola, University of Michigan, USA
Visualization of Transient Interactions Using gelFRET Analysis and Bimolecular Fluorescence Complementation (Bifc)

2:35 PM – 7:00 PM
Free Time

7:00 PM – 9:00 PM
Dinner on Own

Thursday, October 18, 2012

7:00 AM – 12:00 PM
Information Desk

Session 10: Biochemical and In Vivo Methods II
Chair: Erich Wanker, Max Delbruck Center for Molecular Medicine, Germany

8:00 AM – 8:25 AM
Erich Wanker, Max Delbruck Center for Molecular Medicine, Germany
What Small Molecules can do with Aggregation-Prone Polypeptides

8:25 AM – 8:40 AM
Siegfried Musser, Texas A & M University, College Station, USA
Nucleocytoplasmic Transport: Weak Interactions Between Transport Receptors and FG-Nucleoporins Promote Permeability and Rapid Transport

8:40 AM – 8:55 AM
Sumio Terada, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan
Dissecting Slow Axonal Transport Mechanism with Fluorescence Cross-Correlation Spectroscopy

8:55 AM - 9:10 AM
Su Lin, The Biodesign Institute at Arizona State University, USA
Assembly of Photosynthetic Light-Harvesting Complexes in Cells Revealed by Time-Resolved Fluorescence Spectroscopy

9:10 AM – 9:30 AM

Session 11: Proteomic Approaches
Chair: Lan Huang, University of California, Irvine, USA

9:30 AM – 9:55 AM
Lan Huang, University of California, Irvine, USA
Developing Proteomic Approaches to Define Protein-Protein Interactions

9:55 AM – 10:10 AM
Tanja Kortemme, UCSF, San Francisco, USA
A Surprising Promiscuity in Functional Domain-motif Interactions in Mammalian Cells

10:10 AM – 10:25 AM
Johanna Rees, University of Cambridge, Cambridge, UK
SILAC iPAC- a Quantitative Method for Exploring Genuine Components of Protein Complexes and Interactomes by Parallel Affinity Capture

10:25 AM – 10:40 AM
Antony Jackson, Institute of Biophysics, CAS and University of Cambridge, UK
A New Method for the Analysis of Membrane-Bound Protein Assemblies using Tyramide-Biotin Labeling and High Throughput Proteomics

End of meeting


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