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2002
biophysical discussions posters
Posters appear in alphabetical
order, and are presented each day from 4:30 - 6:30 PM
Modeling
the Three-Dimensional Structure of the Yeast Nuclear Pore Complex
Frank Alber, Brian Chait,
Julia Kipper, Mike Rout, Adisetyantari Suprapto, Whenzu Zhang, and Andrej
Sali; The Pels Family Center for Biochemistry and Structural Biology, The
Rockefeller University, New York, NY 10021
Myosin
Head Disposition Modeled from Low-Angle X-Ray Diffraction of Relaxed Lethocerus
Insect Flight Muscle Hind
A. AL-Khayat1, Liam Hudson1,
Michael K Reedy2, Bruce Baumann3, Thomas C Irving4 & John M. Squire1;
1Biological Structure and Function Section, Biomedical Sciences Division,
Imperial College of Science, Technology & Medicine, London SW7 2AZ,
UK, 2Department of Cell Biology, Duke University, Durham, NC 27710, USA,
3Florida State University, Tallahassee, FL, USA, 4Illinois Institute of
Technology, Chicago, IL 60616, USA
Biochemical
probes of microtubule structure: the fast kinetics of Taxol binding
José M. Andreu, I.
Barasoain and J.Fernando Díaz . CIB, CSIC, Madrid, Spain
Advanced
Instrumentation for High Resolution Electron Cryo-Microscopy
B.L. Armbruster1, K. Fukushima2,
M. Kawasaki1, M. Kersker1 and R. O'Donnell1, 1JEOL USA, Inc., Peabody,
MA 01960,
2JEOL Ltd., Akishima, Japan
Electron
Tomography of Hair Cell Stereocilia - Toward a 3D Structure of the Hearing
Machinery
Manfred Auer1,2, Bram
Koster3, Ulrike Ziese3, Da Neng Wang1 & A. James Hudspeth2, 1Skirball
Institute, NYU Medical
Center, 2 Laboratory of
Sensory Neuroscience, The Rockefeller University, 3 University of
Utrecht
Computational
Prediction of Structure-Based Dynamics For Biomolecular Complexes and Assemblies
Ivet Bahar; Center for Computational
Biology & Bioinformatics, and Department of Molecular Genetics &
Biochemistry, School of
Medicine, University of
Pittsburgh, PA 15213, USA
Structural
and Functional Mining of Intermediate Resolution Structures of Biological
Machines
Matthew Baker, Wen Jiang
and Wah Chiu; Department of Biochemistry and Molecular Biology, Baylor
College of Medicine,
Houston, Texas
77030
Towards
Structure Determination of Membrane Proteins in 2-Dimensional Crystals
Using Next-Generation Hard X-Ray Sources
Michael Becker; Biology
Dept., Brookhaven National Laboratory, P.O. Box 5000, Upton, NY
11973
SenSitus–Force
Feedback for Fitting EM and X-tal Data
Stefan Birmanns1 , Willy
Wriggers2; 1Central Institute for Applied Mathematics, Research Centre
Juelich, Germany,
s.birmanns@fz-juelich.de;
2Department of Molecular Biology, The Scripps Research Institute, wriggers@scripps.edu
Conformational
Changes in the Nucleotide Binding Domain of the ABC Transporter RbsA Due
to ATP Hydrolysis
Matthew C. Clifton ‡, Huide
Zhang §, Shelly Armstrong ‡, Mark A. Hermodson §, Cynthia Stauffacher
‡; From the
‡Department of Biological
Sciences and the §Department of Biochemistry, Purdue University, West
Lafayette, Indiana 47906
Towards
Atomic Resolution Structures of Type IV Pili
L. Craig1, A. S. Arvai1,
R. K. Taylor2, M. Pique1, M. Singh3, D.S. Shin1, B. D. Adair4, S. J. Lloyd1,
K. T. Forest5, M.
Yeager4 and J. A. Tainer1;
1The Scripps Research Institute, Dept. of Molecular Biology and 4Dept.
of Cell Biology, La Jolla, CA;
2Dartmouth Medical School,
Dept. of Microbiology, Hanover, NH; 3Albert Einstein College of Medicine,
Bronx, NY; 5University of Wisconsin, Department of Bacteriology, Madison,
WI
Cyanobacterial
Photosystem II and Its Antennae System
Paula C. A. da Fonseca,
Edward P. Morris & James Barber; Biochemistry Department, Imperial
College, London SW7 2AY, U.K.
Cryo-Eecton
Microscopy of Vitreous Sections: Cell Ultrastructure Revisited
Jacques Dubochet, Laboratory
for Ultrastructural Anlaysis, University of Lausanne, CH-1015 Lausanne,
Switzerland and
Alasdaidre McDowall, Centre
for Microscopy and Microanalysis, University of Queensland, St. Lucia,
QLD 4072 Australia.
Structure
of a Bacterial DNA-Protein Complex that Mediates Nuclear Import in Plants
Michael Elbaum1, Asmahan
Abu-Arish1, Sharon Grayer Wolf2 1Dept of Materials and Interaces and 2Electron
Microscopy Center, Weizmann Institute of Science, Rehovot, Israel
Comparative
Protein Structure Models for Molecular Modeling of the Yeast Ribosome
N. Eswar1, C.M.T. Spahn2,
R. Beckmann3,4, P.A. Penczek5, G. Blobel3, J. Frank2,6, A. Sali1; 1Laboratory
of Molecular
Biophysics, Pels Family
Center for Biochemistry and Structural Biology, The Rockefeller University,
New York, NY 10021;
2Wadsworth Center, Howard
Hughes Medical Institute, Empire State Plaza, Albany, New York 12201; 3Laboratory
of Cell
Biology, Howard Hughes Medical
Institute, The Rockefeller University, New York, NY 10021; 4Institut für
Biochemie der
Charité, Humboldt
Universtität zu Berlin, Monbijoustr. 2, 10117, Berlin, Germany.; 5University
of Texas, Houston Medical
School, 6431 Fannin, Houston,
Texas 77030; 6Department of Biomedical Science, State University of New
York at Albany,
Albany, New York 12222.
Crystallographic
Studies of DEDD Family Exoribonucleases
Tristan J. Fiedler &
Arun Malhotra; Department of Biochemistry & Molecular Biology, University
of Miami School of Medicine,
Miami, FL 33101, USA
Structure
of a Stathmin-like Domain: Tubulin Complex
Benoît Giganta, Patrick
A. Curmib, Carole Martin-Barbeya, Raimond Ravellic, André Sobelb
and Marcel Knossowa; aLEBS, CNRS Gif sur Yvette, France bINSERM U 440
Paris, France cEMBL Grenoble, France
Electron
Tomography of Whole Cells and Organelles
Grant Jensen1, Dieter Typke1,
Julia Kuerner2, Tina Weatherby3, and Kenneth Downing1; 1Lawrence Berkeley
National Lab,
2Max Planck Institute for
Biochemistry; 3University of Hawaii Manoa
Three-dimensional
structure of IP3 receptor channel at 24 Å resolution
Qiu-Xing Jiang, Edwin C.
Thrower, David W Chester, Barbara E. Ehrlich and Fred J. Sigworth, Departments
of Cellular and
Molecular Physiology and
Pharmacology, Yale University, 333 Cedar Street, New Haven, CT 06520
Towards
High Throughput and High Resolution 3-D Single Particle Reconstruction
of Macromolecular Complexes
Wen Jiang and Wah Chiu;
Verna and Marrs McLean Department of Biochemistry and Molecular Biology,
Baylor College of
Medicine, Houston, TX 77030
Contrast
Transfer Function - Estimation of Parameters
Laurent Joyeux, Pawel A.
Penczek; Department of Biochemistry and Molecular Biology; University of
Texas–Houston Medical
School, 6431 Fannin, Houston,
TX 77030, USA.
Purification
and structural analysis of native intermediate-containing spliceosomes
Melissa S. Jurica, Melissa
J. Moore, Nikolaus Grigorieff; Howard Hughes Medical Institute/ Biochemistry
Department, Brandeis
University, Waltham, MA
Examining
the Alpha-actinin-b1-Integrin Structrual Relationship Using Cryo-EM
Debbie Kelly, Dianne Taylor,
Kenneth A. Taylor; Institute of Molecular Biophysics, Florida State University,
Tallahassee, FL
32306
Elastic
Conformational Transitions
M. K. Kim1, G. S. Chirikjian1,
Robert L. Jernigan2; 1Department of Mechanical Engineering, The Johns Hopkins
University,
Baltimore, MD 21218; 2Laurence
H. Baker Center for Bioinformatics and Biological Statistics, Iowa State
University, Ames, IA
50011-3020
Optimizing
the Fit of Known Structures into Electron Microscope Images
Andrei Korostolev, Lifan
Chen, Jinghua Tan, Ken Taylor, Felcy Fabiola, Eric Blanc & Michael
S. Chapman; Department of Chemistry & Biochemistry, Department
of Biological Science and Institute of Molecular Biophysics, Florida State
University, Tallahassee, FL 32306-4380
Fast
Rotational Matching
Julio A. Kovacs, Pablo Chacon,
Willy Wriggers; The Scripps Research Institute, Dept. of Molecular Biology,
TPC-6, 10550 N.
Torrey Pines Rd., La Jolla,
CA 92037, jkovacs@scripps.edu,
pchacon@scripps.edu,
wriggers@scripps.edu
Refined
Model of the 1OS Conformation of Smooth Muscle Myosin By Cryoem 3-D Image
Reconstruction
Jun Liu, Dianne W. Taylor,
Thomas Wendt and Kenneth A. Taylor; Institute of Molecular Biophysics,
Florida State University,
Tallahassee, FL 32310
How
we can understand the mechanism expressed by the actin filament, being
based on the atomic structure of the actin filament
complex
Yuichiro Maeda, Soichi Takeda,
Atsuko Yamashita & Kayo Maeda; RIKEN Harima Institute at SPring-8,
Kouto, Mikazuki, Hyogo,
Japan 679-5148; ymaeda@spring8.or.jp
Atomic
Force Microscopy in the Study of Virus Particles, Virus Crystals, and Viral
Infected Cells
A. McPherson, Yu. G. Kuznetsov,
A. J. Malkin, and M. Plomp; University of California, Dept. of Molecular
Biology &
Biochemistry, 560 SH, Irvine,
92697
THREE-DIMENSIONAL
STRUCTURES OF RNA POLyMERASE HOLOENZYME AND THE RNA POLYMERASE-PROMOTER
OPEN
COMPLEX: SYSTEMATIC FLUORESCENCE RESONANCE ENERGY TRANSFER AND DISTANCE-CONSTRAINED
DOCKING
Vladimir Mekler, Ekaterine
Kortkhonjia, Jayanta Mukhopadhyay, Achillefs N. Kapanidis, Andrei Revyakin,
Yon W. Ebright,
Jennifer L. Knight, Ronald
M. Levy, and Richard H. Ebright; Howard Hughes Medical Institute, Waksman
Institute, and
Department of Chemistry
and Chemical Biology, Rutgers University, Piscataway NJ 08854
The
polar T1 interface is linked to conformational changes that open the voltage-gated
potassium channel
Daniel L. Minor, Jr. Department
of Biochemistry and Biophysics, Cardiovascular Research Institute, University
of California, San
Francisco, CA 94131-0130
Molecular
architecture of the protein import channel of the mitochondrial outer membrane
and its larger multi-channel structures
Kirstin Model1, Thorsten
Prinz2, Teresa Ruiz1, Michael Radermacher1, Chris Meisinger2, Nikolaus
Pfanner2 & Werner
Kühlbrandt1, 1Max-Planck
Institute of Biophysics, Department of Structural Biology, Heinrich-Hoffmann-Str.
7, 60528 Frankfurt,
Germany; 2Institute of Biochemistry
and Molecular Biology, University of Freiburg, Hermann-Herder-Str. 7, 79104
Freiburg,
Germany
Multi-resolution
contour-based fitting of macromolecular structures
Pablo Chacon Montes, Dept.
of Molecular Biology, TPC-6, The Scripps Research Institute, 10550 N. Torrey
Pines Road, La Jolla, CA 92037
3D
structure of the CP43' photosystem-I supercomplex of cyanobacteria revealed
by electron cryo-microscopy and single particle
analysis
Edward Morris, Thomas Bibby,
Jonathan Nield and James Barber; Department of Biological Sciences Imperial
College, London
Structural
Basis of Transcription Initiation
K.S. Murakami, S. Masuda,
E.A. Campbell, O. Muzzin, S.A. Darst, The Rockefeller University
Consistency
of 3D reconstructions in single particle analysis
Pawel A. Penczek; Department
of Biochemistry and Molecular Biology; University of Texas–Houston Medical
School, 6431
Fannin, Houston, TX 77030,
USA.
Interaction
of Release Factor RF2 with the Release Complex Visualized by Cryo-Electron
Microscopy
Urmila B. Rawat1, A. Zavialov4,
J. Sengupta2, M. Valle1, R.A. Grassucci1, J. Linde1, B. Vestergaard5, M.
Kjielgaard5, M.
Ehrenberg4, and J. Frank1-3;
1Howard Hughes Medical Institute, 2Wadsworth Center and 3Department of
Biomedical Sciences,
State University of New
York at Albany, Empire State Plaza, Albany, New York 12201-0509, USA; 4Department
of Cell and
Molecular Biology, BMC,
Uppsala University, Box 596, 5-75124 Uppsala, Sweden; 5Institute of Molecular
and Structural
Biology, University of Aarhus,
Denmark.
Quick-freeze
& freeze-substitution of insect flight muscle (IFM) for electron tomography
M. K. Reedy1, M. C. Reedy1,
C. Lucaveche1, Y.E. Goldman2, C. Franzini-Armstrong2 & K.A. Taylor3;
1Cell Biology, Duke
Univ, Durham, NC; 2Penn.
Muscle Inst., Univ Penn, Philadelphia, PA; 3Inst. Molec. Biophys., Fla
State Univ, Tallahassee, FL
A
method for creating a high-resolution projection difference map
Gang Ren, Alok K. Mitra;
Department of Cell Biology, The Scripps Research Institute, MB21, La Jolla,
CA 92037
Crystallographic
Analysis of a 3.6 Million Dalton Respiratory Protein
W.E. Royer & K. Strand,
University of Massachusetts Medical School, Worcester, MA
RECONSTRUCTING
HELICAL OBJECTS USING SINGLE PARTICLE IMAGE PROCESSING, MULTIVARIATE STATISTICAL
ANALYSIS,
AND A TOMOGRAPHIC RECONSTRUCTION ALGORITHM
Rasmus R. Schroeder, Isabel
Angert, Joachim Frank*, Kenneth C. Holmes; Max-Planck Institute for medical
research, Biophysics
Dept., Heidelberg, Germany;
*Howard Hughes Institute, Wadsworth Center, Albany, USA
The
quaternary structure of nitrilases from 2nm negative stain maps and homology
modelling
B.T. Sewell and MN Berman;
University of Cape Town, South Africa
The
structure of the eukaryotic 80S ribosome from S. cerevisiae
Christian M.T. Spahn1, 2,
Roland Beckmann4, 5, Narayanan Eswar7, Pawel A. Penczek6, Andrej Sali7,
Günter Blobel4 and
Joachim Frank1, 2, 3; 1HHMI,
Health Research Inc. at the; 2 Wadsworth Center, Empire State Plaza, Albany,
NY; 3Department
of Biomedical Science, SUNY
at Albany; 4Laboratory of Cell Biology, HHMI, The Rockefeller University,
New York, NY;5Institut
für Biochemie der Charité,
Humboldt Universität zu Berlin, Germany; 6 University of Texas - Houston
Medical School, TX;
7Laboratory of Molecular
Biophysics, The Rockefeller University, New York, NY
EM
analysis of large, multi-subunit transcriptional regulatory complexes reveals
distinct, activator-dependent conformations
Dylan J. Taatjes1, Anders
M. Näär2, Eva Nogales1, and Robert Tjian1; 1Howard Hughes Medical
Institute, Department of
Molecular and Cell Biology
University of California, Berkeley.; 2Department of Cell Biology, Harvard
Medical School
Massachusetts General Hospital
Cancer Center.
Image
reconstruction from cryo-electron micrographs of in vitro assembled tubes
of HIV-1 p24 capsid and Capsid-Nucleocapsid
protein
reveals potential subunit interactions involved in assembly
Dennis R. Thomas*, Jason
Lanman*, Thomas Wilk#, Stephen Fuller# and Peter E. Prevelige*. *Dept.
of Microbiology, University
of Alabama Birmingham, #Division
of Structural Biology, Welcome Trust Center for Human Genetics,Unversity
of Oxford,
Oxford, England
3D
Motifs: Structure & Function Prediction
Jerry Tsai, Texas A&M
University, Department of Biochemistry & Biophysics
AN
ALL-ATOM HOMOLOGY MODEL OF THE E. COLI 30S RIBOSOMAL SUBUNIT
C.-S. Tung, Los Alamos National
Laboratory, K. Sanbonmatsu, Los Alamos National Laboratory, S. Joseph,
UCSD
Interaction
of EF-G with post-termination ribosomes studied by cryo-electron microscopy
M. Valle1, A. Zavialov2,
J. Sengupta3, U. Rawat1, M. Ehrenberg2 & J. Frank1; 1Howard Hughes
Medical Institute, Heath
Research, Inc., 3Wadsworth
Center, Empire State Plaza, Albany, New York 12201-0509, USA; 2Department
of Cell and
Molecular Biology. Biomedical
Center, S-75124 Uppsala, Sweden
Analysis
of heterogeneous complexes by combined statistical analysis and reference
matching
H. White1, Shaoxia Chen1,2,
Brent Gowen3, Johannes Buchner4 , H. Saibil1, E. Orlova1; 1School of Crystallography,
Birkbeck
College, London, UK, 2Dept
of Bioscience, Imperial College, London, UK, 3Wellcome Trust Centre for
Human Genetics,
Oxford, UK, 4Institut für
Organische Chemie und Biochemie, Technische Universität, München,
Garching, Germany
Stress-Induced
Protein/DNA Crystalline Arrays observed in-situ by Electron Tomography
Sharon Grayer Wolf*,
Daphna Frenkiel-Krispin#, Eyal Shimoni* and Abraham Minsky#; *Electron
Microscopy Center, #Organic
Chemistry Department, Weizmann
Institute of Science, Rehovot 76100 ISRAEL
A
method to study the structure of functional channels and transporters in
their native environment
G.A. Zampighi1-2, S. Lanzavecchia3,
M. Kreman1, L. Zampighi2, E. Turk2 and E. Wright2. Departments of
Neurobiology1 and
Physiology2, UCLA School
of Medicine. Dipartimento di Chimica Strutturale3, Universita di Milano
Structure
of Dengue Virus: Implications for Flavivirus Organization, Maturation,
and Fusion
Wei Zhang*, Richard J. Kuhn*,
Michael G. Rossmann*, Sergei V. Pletnev*, Jeroen Corver#, Edith Lenches#,
Christopher T.
Jones*, Suchetana Mukhopadhyay*,
Paul R. Chipman*, Ellen G. Strauss#, Timothy S. Baker*, & James H.
Strauss#;
*Department of Biological
Sciences, Purdue University, West Lafayette, IN 47907-1392,
USA. # Division of Biology 156-29,
California Institute of
Technology, Pasadena, CA 91125, USA.
MIRS:
a High-Resolution 3D Reconstruction Package Integrated with a Relational
Image Database
Z. Hong Zhou, Eugene Y.
Ke, Yuyao Liang; Department of Pathology and Laboratory Medicine, University
of Texas - Houston
Medical School, Houston,
TX 77030, USA
Three-Dimensional
Organization of the Human Platelet Integrin aIIbb3
Brian D. Adair and Mark
Yeager, The Scripps Research Institute
Crystal
Structure of the Upper Domain of VP5, the Major Nucleocapsid Protein of
Herpes Simplex Virus-1
Brian R. Bowman, Baylor
College of Medicine, Texas
Arp2/3
complex promotes actin filament assembly at the leading edge of motile
cells: insights at atomic resolution
Thomas D. Pollard. Molecular,
Cellular and Developmental Biology, Yale University, New Haven CT.
High
Resolution Imaging of Single Particles in a 300 kV Liquid Helium Electron
Cryomicroscope
Zongli Li and Wah Chiu;
National Center for Macromolecular Imaging, Baylor College of Medicine,
One Baylor Plaza, Houston,
TX 77030, USA |
