2006 Biophysical Society Discussions Study Book - Posters

Posters 1 - 51 will be displayed from Thursday 8:00 pm until Friday 11:00 pm and presented in Poster Session I on Friday, October 20th from 2:00 to 5:00 pm.

Posters 52 - 104 will be will be displayed from Saturday 8:00 am until Sunday 11:00 am and presented in Poster Session II on Saturday, October 21st from 2:00 to 5:00 pm.

Poster Session I

Force-generating Mechanisms & Motor Walking Mechanisms

Poster Session I Review

Sarah Rice, Northwestern University

Poster 1

The beginning of kinesin's force-generating cycle visualized at 9A resolution.

Charles V. Sindelar, Ph.D., Kenneth Downing, Ph.D. Life Sciences Division, Lawrence Berkeley National Labs, Berkeley, CA, USA.

Poster 2

The Dam1 kinetochore ring complex harnesses microtubule dynamics to produce force and movement.

Charles L. Asbury¹, Trisha N. Davis², Daniel R. Gestaut², Andrew F. Powers¹, Andrew D. Franck¹.
¹Physiology and Biophysics, University of Washington, Seattle, WA, USA, ²Biochemistry, University of Washington, Seattle, WA, USA.

Poster 3

A structural model for monastrol inhibition of dimeric kinesin Eg5.

Troy C. Krzysiak¹, Thomas Wendt², Lisa R. Sproul¹, Heinz Gross³, Susan P. Gilbert¹, Andreas Hoenger⁴.
¹Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA, ²European Molecular Biology Laboratory, Heidelberg, Germany, ³Electron Microscopy ETH Zurich (EMEZ) c/o Institution for Applied Physics, Zuerich-Hoenggerberg, Switzerland, ⁴University of Colorado at Boulder, Boulder, CO, USA.

Poster 4

Dimeric Eg5/KSP Exhibits Alternating Site ATP Catalysis

Troy C. Krzysiak, B.S, Susan P. Gilbert, Ph.D. Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA.

Poster 5

The torque-speed relationship of the bacterial flagellar motor.

Jianhua Xing, Ph.D.¹, Fan Bai², Richard Berry², George Oster³. ¹Physical Biosciences Institute, Lawrence Livermore National Laboratory, Livermore, CA, USA, ²Physics, University of Oxford, Oxford, United Kingdom, ³University of California, Berkeley, CA, USA.

Poster 6

Regulation of Myosin VI’s ADP Affinity by Load.

Sergey Mikhailenko¹, Yusuke Oguchi¹, Adrian O. Olivares², Enrique M. De La Cruz², Shin’ichi Ishiwata¹. ¹Department of Physics, School of Science and Engineering, Waseda University, Tokyo, Japan, ²Molecular Biophysics & Biochemistry Department, Yale University, New Haven, CT, USA.

Poster 7

Diffusion of Matrix Metalloproteases on the Surface of Collagen Fibrils: The Mobile Cell Surface - Collagen Substratum Interface Hypothesis.

Gregory I. Goldberg, PhD¹, Ivan E. Collier, PhD², Saveez Saffarian, PhD³, Elliot Elson, PhD⁴. ¹Medicine, Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, MO, USA, ²Medicine, Washington University School of Medicine, Saint Louis, MO, USA, ³Cell Biology, Harvard Medical School, Boston, Ma, MA, USA, ⁴Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, MO, USA.

Poster 8

Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro

Megan T. Valentine, Ph.D.¹, Polly M. Fordyce², Troy C. Krzysiak³, Susan P. Gilbert³, Steven M. Block⁴. ¹Biological Sciences, Stanford University, Stanford, CA, USA, ²Physics, Stanford University, Stanford, CA, USA, ³Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA, ⁴Biological Sciences & Applied Physics, Stanford University, Stanford, CA, USA.

Poster 9

The Rotor Ring of Chloroplast ATP Synthase

Ben Varco-Merth, PhD Candidate. Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA.

Poster 10

Mechanisms of poleward chromosome movement

Ekaterina L. Grishchuk¹, Maxim I. Molodtsov¹,², Artem K. Yefremov¹,², Ilia S. Spiridonov¹,², Fazly I. Ataullakhanov²,³, J. Richard McIntosh¹. ¹MCD Biology, University of CO, Boulder, CO, USA, ²Natl. Res. Cntr. Hematology, Moscow, Russian Federation, ³Physics Dept., Moscow State Univ., Moscow, Russian Federation.

Poster 11

Requirements for Yeast Cytoplasmic Dynein Processivity

Samara L. Reck-Peterson, PhD, Andrew Carter, PhD, Arne Gennerich, PhD, Ronald Vale, PhD. Cellular and Molecular Pharmacology, UCSF, San Francisco, CA, USA.

Poster 12

Force-production and stepping-mechanism of cytoplasmic dynein

Arne Gennerich, Ph.D., Andrew Carter, Ph.D., Sam Reck-Peterson, Ph.D., Ahmet Yildiz, Ph.D., Ron Vale, Ph.D. Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA, USA.

Poster 13

Coordination Between the Actin-Binding Cleft and Active Site of Smooth Muscle Myosin.

Christopher L. Berger, Ph.D.¹, Lynn R. Chrin, B.S.¹, Donald P. Gaffney II, B.S.¹, C. Ian Robertson, Ph.D.¹, Jared Cochran, Ph.D.², Susan P. Gilbert, Ph.D.². ¹Molecular Physiology & Biophysics, University of Vermont, Burlington, VT, USA, ²Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA.

Poster 14

Are the structures of F-actin and the F-actin backbone in the strongly bound actin-myosin Rigor complex different?

Rasmus R. Schroeder¹, Werner Jahn², Kenneth C. Holmes². ¹Structural Biology, Max-Planck-Institute of Biophysics, 60438 Frankfurt am Main, Germany, ²Max-Planck-Institute for Medical Research, 69120 Heidelberg, Germany.

Poster 15

A detailed kinetic analysis of the cardiac myosin activator, CK-1213296, suggests it improves cardiac contractility by accelerating transition from the weak to strongly bound states.

James J. Hartman, Ph.D.¹, Hector M. Rodriguez, Ph.D.¹, Sheila Sylvester, B.S.¹, Y T. Shen, M.D.², David A. Kass, M.D.³, Stevphen F. Vatner, M.D.², Fady Malik, M.D., Ph.D.⁴, Roman Sakowicz, Ph.D.¹. ¹Biochemistry, Cytokinetics, Inc., South San Francisco, CA, USA, ²Department of Cell Biology & Molecular Medicine, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA, ³Johns Hopkins, Baltimore, MD, USA, ⁴Cytokinetics, Inc., South San Francisco, CA, USA.

Poster 16

Phosphorylation of the Myosin Regulatory Light Chain Affects the Conformation and Affinity for ADP of the Nucleotide Pocket

Roger Cooke, PhD¹, Nariman Naber, PhD¹, Tomas Purcell, PhD¹, Edward Pate, PhD². ¹biochem/Biophys, Univ california, San Francisco, CA, USA, ²Applied Mathematics, Washington State Univ., Pullman, WA, USA.

Poster 17

Large Conformational Changes in a Kinesin Motor Catalysed by Interaction with Microtubules

Keiko Hirose, Ph.D.¹, Toshihiko Akiba, Ph.D.², Sharyn A. Endow, Ph.D.³, Linda A. Amos, Ph.D.⁴. ¹Research Institute for Cell Engineering, AIST, Tsukuba, Ibaraki, Japan, ²Biological Information Research Center, AIST, Tsukuba, Ibaraki, Japan, ³Department of Cell Biology, Duke University Medical Center, Durham, NC, USA, ⁴MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.

Poster 18

Dissection of Intra-molecular Communications between the Catalytic Head and Microtubule-binding Site in the Dynein Heavy Chain.

Takahide Kon, Ph.D., Kenji Imamula, Reiko Ohkura, Kazuo Sutoh, Ph.D. Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan.

Poster 19

Loading direction controls the ADP affinity of myosin V

Yusuke Oguchi¹, Sergey V. Mikhailenko¹, Adrian O. Olivares², Enrique M. De La Cruz², Shin'ichi Ishiwata¹. ¹Dept.Phys, Waseda Univ, Tokyo, Japan, ²Molecular Biophysics & Biochemistry Department, Yale University, New Haven, CT, USA.

Poster 20

A Kinetic Study of the CENP-E ATPase

Marilyn van Duffelen, Ph.D., Steven S. Rosenfeld, MD, PhD.  Neurology, Columbia University Medical Center, New York, NY, USA.

Poster 21

Defining Elements of Yeast Cytoplasmic Dynein Required for Motility

Andrew P. Carter, PhD, Samara L. Reck-Peterson, PhD, Ahmet Yildiz, PhD, Arne Gennerich, PhD, Ronald D. Vale, PhD.
Cellular and Molecular Pharmacology, UCSF, San Francisco, CA, USA.

Poster 22

Actin Nucleators Working Together - or - Spire keeps Surprising Us.

Margot E. Quinlan¹, Eugen Kerkoff², R. Dyche Mullins^{1 1}Dept. of Cellular and Molecular Pharmacology, University of California San Francisco, ²BayGene and MSZ, Universität Würzburg, Germany.

Poster 23

Myosin S1 structure revealed by multifrequency EPR and site-directed spin labeling

Yuri E. Nesmelov¹, Valentin V. Novikov¹,², Adam Burr¹, Roman Agafonov¹, David D. Thomas¹. ¹Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA, ²Engelhardt Institute of Molecular Biology RAS, Moscow, Russian Federation.

Poster 24

Structural modeling of the N-terminal domain of the essential myosin light chain and its functional analysis in the intact heart.

Ewald Mirko Aydt, PhD¹, Hannelore Haase, PhD², Gilla Tünnemann², Cristina Cardoso, PhD², Gerhard Wolff, PhD¹, Ingo Morano, PhD². ¹Revotar Biopharmaceuticals, Hennigsdorf, Germany, ²Molecular Muscle Physiology, Max-Delbrueck-Center for Molecular Medicine, Berlin, Germany.

Poster 25

Roles of multiple nucleotide-binding/hydrolysis sites of the cytoplasmic dynein motor domain in microtubule sliding

Kazuo Sutoh, Toshifumi Mogami, Kenji Imamula, Tomohiro Shima, Reiko Ohkura, Takahide Kon. Dept Life Sciences, University of Tokyo, Tokyo, Japan.

Poster 26

Why can Acto-S1 chimera protein filaments move on a myosin-coated surface as fast as skeletal F-actin, and why do water molecules around F-actin become more mobile than bulk water upon interaction with S1?

Makoto Suzuki, Professor¹,², Takashi Miyazaki¹,², Md.Shahjahan Parvez Siddique, Dr.Eng.¹, Eisaku Katayama, Dr.Sci.³, Taro Q.P. Uyeda, Dr.Sci.⁴, George Mogami¹, Syed Rashel Kabir, Dr.Eng.5, Tetsuichi Wazawa, Dr.Sci.6.
 

Poster 27

Mechanochemical coupling in myosin motor domain: insights from combined potential of mean force, molecular dynamics, normal mode and statistical coupling analysis.

Qiang Cui, Ph.D. Chemistry, Univ. of Wisc., Madison, Madison, WI, USA.

Poster 28

Temperature change does not alter single molecular force generated between the regulated actin filament and HMM

Masataka Kawai, PhD¹, Takanori Kido, MS², Martin Vogel, PhD³, Rainer HA Fink, PhD³, Shin'ichi Ishiwata, PhD².
¹Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USA, ²Physics, Waseda University, Tokyo, Japan, ³Medical Biophysics Unit, Institute of Physiology and Pathophysiology, Ruprecht-Karls-Universität, Heidelberg, Germany.

Poster 29

Mechanistic consequences of movement on two-state tension generation in skeletal muscle

Julien S. Davis, Neal D. Epstein. Molecular Cardiology, NIH, Bethesda, MD, USA.

Poster 30

Identifying the Power Stroke Step of Myosin V Using Computational Dwell-Time Distribution Analysis

Jung-Chi Liao, PhD¹, David Parker², Thomas J. Purcell, PhD³, James A. Spudich, PhD⁴, Scott L. Delp, PhD¹. ¹Bioengineering, Stanford University, Stanford, CA, USA, ²Mechanical Engineering, Stanford University, Stanford, CA, USA, ³Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA, ⁴Biochemistry, Stanford University, Stanford, CA, USA.

Poster 31

Dynamic polymorphism of single actin molecules in the actin filament.

Hiroaki Yokota¹, Jun Kozuka²,³, Yoshiyuki Arai²,³, Yoshiharu Ishii², Toshio Yanagida²,⁴. ¹Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan, ²Formation of Soft Nanomachines, Core Research for Evolution Science and Technology, Japan Science and Technology Agency, Suita, Osaka, Japan, ³Department of Biophysical Engineering, Osaka University, Suita, Osaka, Japan, ⁴Department of Biophysical Engineering & Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.

Poster 32

Development of a high-pressure microscope and its application to kinesin-microtubule complex.

Masayoshi Nishiyama, Ph.D¹, Yoshifumi Kimura, Ph. D², Yoshio Nishiyama¹, Masahide Terazima, Ph.D¹. ¹Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, Japan, ²International Innovation center, Kyoto University, Kyoto, Japan.

Poster 33

Step Size of Kinesin Measured by Electron Paramagnetic Resonance.

Kazunori Sugata¹, Likai Song², Motoyoshi Nakamura¹, Shoji Ueki¹, Piotr G. Fajer², Toshiaki Arata¹. ¹Dept Biol Sci, Osaka University, Toyonaka, Osaka, Japan, ²Dept Biol Sci and NHMFL, Florida State University, Tallahassee, FL, USA.

Poster 34

Simultaneous observation of rotatory and stepping motions of single myosin II molecules.

So Nishikawa¹, Toshio Yanagida². ¹JST, CREST, Osaka, Japan, ²Osaka university, Osaka, Japan.

Poster 35

A New Structural Model Reveals Energy Transduction in Dynein.

Adrian W. R. Serohijos¹, Yiwen Chen¹, Feng Ding, PhD², Timothy C. Elston, PhD³, Nikolay V. Dokholyan, PhD².
¹Physics Department and Program in Molecular and Cellular Biophysics, University of North Carolina-Chapel Hill, Chapel HIll, NC, USA, ²Department of Biochemistry and Biophysics, University of North Carolina-Chapel Hill, Chapel HIll, NC, USA, ³Department of Pharmacology, University of North Carolina-Chapel Hill, Chapel HIll, NC, USA.

Poster 36

Conformational Dynamics of the Nucleotide Binding Site in Fast and Slow Muscle Fibers.

Nariman Naber, Ph.D¹, Tom Purcell, Ph.D², Edward Pate, Ph.D³, Roger Cooke, Ph.D⁴. ¹Biochem, UCSF, San francisco, CA, USA, ²CVRI, UCSF, San francisco, CA, USA, ³Math, Washington State University, Pullman, WA, USA, ⁴Biochem and Biophysics and CVRI, UCSF, San francisco, CA, USA.

Poster 37

The role of strong actin-myosin binding in muscle force and motion generation.

Josh E. Baker, PhD. Biochemistry, University Nevada Reno, Reno, NV, USA.

Poster 38

Nucleotide Pocket Conformation Dynamics In Cardiac Muscle Myosin

Thomas J. Purcell, Ph.D.¹, Nariman Naber, Ph.D.¹, Kathy Franks-Skiba¹, Edward Pate, Ph.D.², Roger Cooke, Ph.D.¹.
¹Biochemistry, University of California, San Francisco, San Francisco, CA, USA, ²Math, Washington State University, Pullman, WA, USA.

Poster 39

Mechanics and Structural Dynamics of Muscle Myosin Underlying the Force-Velocity Relation.

G. Piazzesi¹, M. Linari¹, M. Reconditi¹,  P. Bianco¹, E. Brunello¹, A. Stewart², T. Irving³, D. Gore³, M. Irving⁴ and V. Lombardi¹.

¹Laboratorio di Fisiologia, DBAG, Università di Firenze, Italy;  ²Dexela Ltd., London UK; ³BioCAT, Advanced Photon Source, Argonne, USA; ⁴Randall Division of Cell and Molecular Biophysics, King’s College London UK.

Poster 40

Force generation mechanism of axonemal dynein inspired by the structure of isolated and in situ dyneins of Chlamydomonas axonemes

Kazuhiro Oiwa, Prof.¹,², Hitoshi Sakakibara, Ph.D.¹, Takashi Ishikawa, Ph.D.³.
¹Kobe Advanced ICT Research Center, Kobe, Japan, ²Graduate School of Life Science, University of Hyogo, Harima, Japan, ³Department of Biology, Swiss Federal Institute of Technology, Zürich, Switzerland.

Poster 41

Emergent Properties of Actin Organization from Stochastic Simulation of the Dendritic Nucleation Model

Edwin Taylor. University of Chicago and Northwestern Univ., Chicago, IL, USA.

Poster 42

ATPase Mechanism of Eg5 in the Absence of Microtubules

Jared C. Cochran, Ph.D.¹, Susan P. Gilbert, Ph.D.². ¹Chemistry, Dartmouth College, Hanover, NH, USA, ²Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA.

Poster 43

Structure of the Microtubule-Kinetochore Interface Visualized by EM Tomography

Richard McIntosh, Ph.D. Mol. Cell and Devel. Biology, University of Colorado, Boulder, CO, USA.

Poster 44

The Role of Fluid Surface Energy in Muscle Contraction and Motility - Was Szent-Györgyi Right After all?

Alex Metherell, PhD, MD. Donald Bren School of Information & Computer Science, University of California Irvine, Irvine, CA, USA.

Poster 45

Functional Mechanism of Eg5, a Tetrameric Kinesin.

Christoph F. Schmidt¹, Mikhail J. Korneev², Stefan  Lakaemper¹, Benjamin H. Kwok³, Tarun M. Kapoor⁴. ¹Fakultät für Physik, Georg-August-Universität, Gottingen, Germany, 2Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, 3Rockefeller University, New York, NY, USA.
 

Poster 46

Probe conformational transitions and dynamical couplings with normal modes of coarse-grained models

Wenjun Zheng, PhD, Bernard R. Brooks. NIH, Bethesda, MD, USA.

Poster 47

Mechanism of an Unconventional Kinesin-3 Motor

Guenther Woehlke, Sarah Adio, Bettina Ebbing. Adolf-Butenandt-Inst. Cell Biology, Munich, Germany.

Poster 48

Interaction between Motor Heads Strongly Effects the Dynamics and Biophysical Properties of Motor Proteins

Anatoly B. Kolomeisky, Rahul Das. Chemistry, Rice University, Houston, TX, USA.

Poster 49

Mechanism and Energy Coupling of Kinesin ATPases

David D. Hackney. Department of Biological Sciences, Carnegie Mellon Univ., Pittsburgh, PA, USA.

Poster 50

Single Molecule Observations of Structural Changes in the Neck Linker of Kinesin

Michio Tomishige¹, Ronald D. Vale². ¹Department of Applied Physics, University of Tokyo, Tokyo, Japan, ²Department of Molecular and Cellular Pharmacology, University of California, San Francisco, San Fransicso, CA, USA.

Poster 51

Structural studies of microtubule-based motors.

Masahide Kikkawa. Cell Biology, Univ. of Texas, Southwestern, Dallas, TX, USA.

Poster Session II

Motor Walking Mechanisms, Motor Directionality, Motor Regulation, Motors in the Cell

Poster Session II Review

Meredith Betterton, University of Colorado, Boulder

Poster 52

Mechanistic Studies on a Viral DNA Packaging Motor Complex

Carlos Enrique Catalano, Pharm.D., Ph.D.¹, Qin Yang, Ph.D.², Nasib Karl Maluf, Ph.D.³, Helene Gaussier, Ph.D.². ¹University of Washington School of Pharmacy, Seattle, WA, USA, ²Pharmaceutical Sciences, University of Colorado School of Pharmacy, Denver, CO, USA, ³Microbiology, University of Iowa, Iowa City, IA, USA.

Poster 53

Functions of Molecular Motors in vivo

Michelle Peckham¹, Sarah Dunn¹, Ewan Morrison², Rob Cross³. ¹University of Leeds, Leeds, United Kingdom, ²St James, CRUK, Leeds, United Kingdom, ³MCRI Molecular Motors Group, Marie Curie Institute, Oxted, United Kingdom.

Poster 54

Mechanisms of Vesicle Transport Deduced from FIONA Measurements in Living cells.

Igor Kulic¹, Comert Kural², Philip Nelson, PhD¹, Paul Selvin, PhD². ¹Physics/Astronomy, Univ Pennsylvania, Philadelphia, PA, USA, ²Physics, Univ Illinois, Urbana, IL, USA.

Poster 55

Kinesin/Microtubule (un)binding Dynamics depends on the Bulk Kinesin Concentration.

Giovanni Cappello¹, Wouter Roos¹, Otger Campas¹, Fabien Montel¹, Patricia Bassereau¹, Günther Woehlke². ¹Physico-Chimie-Curie, Insitut Curie, Paris Cedex 05, France, ²Zellbiologie, Adolf-Butenandt-Institut (Ludwig-Maximilians-Universität München), D-80336 München, Germany.

Poster 56

Chiral Discrimination by Topoisomerase IV: Crossing Angle and Processivity

Keir C. Neuman, Gilles Charvin, Vincent Croquette, David Bensimon. Physique Statistique, Ecole Normale Superieure, Paris, France.

Poster 57

Minimum Requirements for Processivity in Kinesin-1

Sarah Adio, Guenther Woehlke. Department of Cell Biology, University of Munich (LMU), Munich, Germany.

Poster 58

Electron-Microscopy and Fluorescence Polarization Studies on Motors of the Kinesin Superfamily.

Hernando J. Sosa, Dongyan Tan, Ana Belen Asenjo, Yonatan Weinberg, Vito Mennella, David Sharp. Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY, USA.

Poster 59

The Mechanics of Myosin V

Michael A. Riegelman¹,², James R. Sellers, Ph.D.², Haim H. Bau, Ph.D.¹. ¹Mechanical Engineering & Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA, ²Molecular Physiology, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA.

Poster 60

Interaction of Dynein and Microtubules

Yoko Y. Toyoshima, PhD. Lifesciences, The University of Tokyo, Tokyo, Japan.

Poster 61

Microtubules and Microtubule-binding Proteins studied with Atomic Force Microscopy

Iwan A.T. Schaap, PhD¹,², Carolina Carrasco³, Bernd Hoffmann, PhD⁴, Pedro J. de Pablo, PhD³, Frederick C. MacKintosh, PhD², Christoph F. Schmidt, PhD²,5. ¹Physical biochemistry, NIMR, London, United Kingdom, ²Department of Physics and Astronomy, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands, ³Departamento de Física de la Materia Condensada C-III, Universidad Autónoma de Madrid, Madrid, Spain, ⁴Institute of Thin Films and Interfaces, Research Center Jülich, Jülich, Germany, 5III. Physikalisches Institut, Faculty of Physics, Georg-August-Universität, Göttingen, Germany.

Poster 62

Tracking of Single Kinesin-1 Motor Molecules in Live Cells

Dawen Cai¹, Kristen J. Verhey², Edgar Meyhofer³. ¹Biophysics Research Division, University of Michgan, Ann Arbor, MI, USA, ²Cell and Developmental Biology, University of Michgan, Ann Arbor, MI, USA, ³Mechanical Engineering and Biomedical Enginerring, University of Michgan, Ann Arbor, MI, USA.

Poster 63

The Effect of Monastrol on EG5/DMKHC Chimeras

Stefan Lakämper¹,², Mikahil Korneev², Christoph F. Schmidt¹,². ¹Drittes Physikalisches Institut, Georg-August-Univerität Göttingen, Göttingen, Germany, ²Physics of Complex Systems, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.

Poster 64

Motility and Processivity of Kinesin-2 Motors

William O. Hancock, PhD, Yangrong Zhang, PhD, Gayatri Muthukrishnan, MS. Bioengineering, Pennsylvania State University, University Park, PA, USA.

Poster 65

Experimental Realization of Molecular Motor Models

Benjamin J. Lopez, B.S Physics completed, PhD in progress, Erin Craig, B.S Physics completed, PhD in progress, Heiner Linke, PhD. Physics, University of Oregon, Eugene, OR, USA.

Poster 66

Kinesin Motion induced by Nucleotide Analogs Reveals a Mechanism for Head Coordination

Nicholas R. Guydosh¹, Steven M. Block². ¹Biophysics, Stanford University, Stanford, CA, USA, ²Biological Sciences and Applied Physics, Stanford University, Stanford, CA, USA.

Poster 67

Polymer-based Brownian Motors with Application to Biomolecular Systems

Martin J. Zuckermann, D. Phil. Physics, Simon Fraser University, Burnaby, BC, Canada.

Poster 68

Tracking Single Gold Nanoparticle-Myosin V Conjugates Using Darkfield Imaging.

Alexander Robert Dunn, Ph.D., L. Stirling Churchman, B.S., Zev Bryant, Ph.D., James A. Spudich, Ph.D.. Biochemistry, Stanford University, Stanford, CA, USA.

Poster 69

Myosin VI Walks Wiggly on Actin Filaments

Yujie Sun¹, Harry W. Schroeder¹, John F. Beausang¹, Kazuaki Homma², Mitsuo Ikebe², Yale E. Goldman¹. ¹Physiology, Pennsylvania Muscle Institute, Philadelphia, PA, USA, ²University of Massachusetts, North Worcester, MA, USA.

Poster 70

Processive Run Lengths Of Myosin V Are Determined By Its Affinity For Actin In The Weak-Binding State

Alex R. Hodges, Elena B. Krementsova, Kathleen M. Trybus. Molecular Physiology and Biophysics, University of Vermont, Burlington, VT, USA.

Poster 71

Toward a Coarse-Grained Model of the Myosin V Neck Using the Normal Mode Analysis

David Parker, masters science. Mechanical engineering, stanford university, Stanford, CA, USA.

Poster 72

Regulation of Microtubule-binding Affinity in Dynein

Ian R. Gibbons, Joan E. Garbarino, Andrew Hahn. Mol/Cell Biology, University of California Berkeley, Berkeley, CA, USA.

Poster 73

Single Alpha Helices (SAH domains) in Myosin Motor Function

Peter J. Knight¹, Kavitha Thirumurugan¹, Thomas Baboolal¹, Arnout P. Kalverda¹, Yuhui Xu², Fei Wang², Walter F. Stafford III³, James R. Sellers², Michelle Peckham⁴. ¹Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, United Kingdom, ²NHLBI, National Institutes of Health, Bethesda, MD, USA, ³Analytical Ultracentrifugation Research laboratory, Boston Biomedical Research Institute, Boston, MA, USA, ⁴Institute of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.

Poster 74

Step Sze Adaptations in Myosins V, VI and X.

Ronald S. Rock, Ph.D. Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA.

Poster 75

Understanding Stall, Superstall, Back Steps and Dwell Times

Michael E. Fisher. University of Maryland, College Park, MD, USA.

Poster 76

Mechanical Model for Coordinated, Processive Transport of Dimeric Motors

Erin M. Craig¹, Ben J. Lopez¹, Martin J. Zuckermann², Heiner Linke¹. ¹Physics Dept. and Materials Science Institute, University of Oregon, Eugene, OR, USA, ²Department of Physics, Simon Fraser University, Burnaby, BC, Canada.

Poster 77

Diffusion-limited Processes in Myosin Function

P. Bryant Chase, Ph.D.¹, Nicolas M. Brunet, B.S.², Goran Mihajlović, B.S.³. ¹Biological Science, Molecular Biophysics, Chem & Biomed Engr, Florida State University, Tallahassee, FL, USA, ²Biological Science, Molecular Biophysics, Florida State University, Tallahassee, FL, USA, ³Physics, Center for Materials Research & Tech (MARTECH), Florida State University, Tallahassee, FL, USA.

Poster 78

The Power Stroke of Myosin VI

Zev Bryant, David Altman, James A. Spudich. Biochemistry, Stanford University, Stanford, CA, USA.

Poster 79

Function and Regulation of Cin8p, the Mitotic S. cerevisiae kinesin-5 Homologue

Adina Gerson, M.Sc.¹, Rachel Avunie, M.Sc.², Natalia Movshovich, B.Sc.¹, Irena Gertsberg, Ph.D.², Vladimir Fridman, B.Sc.², Scot C. Kuo, Ph.D.³, Myles Andrew Hoyt, Ph.D.⁴, Benjamin Katz, Ph.D.5, Larisa Gheber, Ph.D.¹. ¹Clinical Biochemistry and Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel, ²Clinical Biochemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel, ³Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA, ⁴Biology, Johns Hopkins University, Baltimore, MD, USA, 5Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Poster 80

Regulation of Myosin V at the Dingle-Molecule Level

Hailong Lu, Elena B. Krementsova, Kathleen M. Trybus. Dept. of Molecular physiology and biophysics, University of Vermont, Burlington, VT, USA.

Poster 81

The Insertion L5 loop in Human Eg5: Is there a Switch III in Kinesin-5 Motor Proteins?

Sunyoung Kim¹, Edward J. Wojcik¹, Richard A. Walker². ¹Biochemistry & Molecular Biology, LSU Health Sciences Center, New Orleans, LA, USA, ²Biological Sciences, Virginia Polytechnic Institute & State University, Blacksburg, VA, USA.

Poster 82

Structure of Human Kip3d - Unconventional Microtubule Depolymerizing Kinesin

Katjusa Brejc, Lisa Belmont, Ming Yu, Yan Lee, Jim Hartman, Jun Guo, Serge Lichtsteiner, Kenneth Wood, Roman Sakowicz. Cytokinetics Inc, South San Francisco, CA, USA.

Poster 83

Structural Basis for the Regulation of Myosin V

James R. Sellers, Ph.D.¹, Takeshi Sakamoto, Ph.D.¹, John A. Hammer, Ph.D.², Kavitha Thirumurugan, Ph.D.³, Peter J. Knight, Ph.D.³. ¹Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA, ²Laboratory of Cell Biology, National Heart, Lung and Blood Institute, Bethesda, MD, USA, ³Institute of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.

Poster 84

Mechanism of Regulation of Myosin-I by Calcium and Calmodulin

John H. Lewis, Joseph M. Laakso, Tianming Lin, E. Michael Ostap. Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.

Poster 85

Molecular Interaction Techniques for Investigating Molecular Motor Interactions with Tubulin and Anti-mitotic Drugs.

John J. Correia, PhD¹, P. Holland Alday, BS¹, Tom Laue, PhD², Walter F. Stafford, PhD³. ¹Biochemistry, Univ. Miss. Medical Center, Jackson, MS, USA, ²Biochemistry and Molecular Biology, Univ. New Hampshire, Durham, NH, USA, ³Analytical

Ultracentrifugation Research Laboratory, Boston Biomedical Research Institute, Watertown, MA, USA.

Poster 86

The Human Kinesin Eg5 as a Potential Target for Drug Development

Frank Kozielski, Dr. Laboratoire des Moteurs Moléculaires, Institut de Biologie Structurale, Grenoble, France.

Poster 87

Biosynthetic Tools to Investigate Cooperativity in Biomotor-Protein Assemblies

Michael Richard Diehl. Bioengineering, Rice University, Houston, TX, USA.

Poster 88

Properties of Multiple-Motor based Transport and Avenues for its Regulation

Michael D. Vershinin, PhD¹, Brian C. Carter, MS¹, David S. Razafsky², Stephen J. King, PhD², Steven P. Gross, PhD¹. ¹Developmental and Cell Biology, UC Irvine, Irvine, CA, USA, ²School of Biological Sciences, University of Missouri - Kansas City, Kansas City, MO, USA.

Poster 89

Phosphorylation-induced Structural Changes in Smooth Muscle Regulatory Light Chain

David Kast J. Kast, L. Michel Espinoza-Fonseca, Sarah E. Blakely, David D. Thomas.
Physics, University of Minnesota, Minneapolis, MN, USA.

Poster 90

Regulation of Kinesin-1 by Folding

David Hackney. Carnegie Mellon Univ., Pittsburgh, PA, USA.

Poster 91

Do 2 or 3 Kinesin Motors Pull A Single Load Faster Than 1 Motor?

David B. Hill¹,², George Holzwarth¹. ¹Physics, Wake Forest University, Winston-Salem, NC, USA, ²Cystic Fibrosis Center, University of North Carolina, Chapel Hill, NC, USA.

Poster 92

Dynamic Cortical Rearrangements, Mechanosensing, and Mechanical Feedback of Living Cells

Douglas N. Robinson, Ph.D. Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Poster 93

Two Point Mutations Near the Myosin Nucleotide-Binding Pocket Differentially Affect ATPase, Actin Motility, Muscle Structure and Physiology

Corey M. Dambacher, B.S.¹, Anthony Cammarato, Ph.D.¹,², Karen Occor, Ph.D.², Aileen F. Knowles, Ph.D.³, William A. Kronert, B.S.¹, Rolf Bodmer, Ph.D.², Sanford I. Bernstein, Ph.D.¹. ¹Biology, San Diego State University, San Diego, CA, USA, ²Burnham Institute, La Jolla, CA, USA, ³Chemistry, San Diego State University, San Diego, CA, USA.

Poster 94

Quantized Intracellular Transport Velocities: Discrete Numbers of Kinesin and Dynein?

Jed C. Macosko, Ph.D., George Holzwarth, Ph.D.. Physics, Wake Forest, Winston-Salem, NC, USA.

Poster 95

X-ray Interference Studies of the Configuration of the Myosin Heads during Steady Shortening of Muscle.

Hugh E. Huxley, Sc.D. Rosenstiel, Brandeis Univ., waltham, MA, USA.

Poster 96

Myo1c Binds Phosphoinositides through a Putative PH Domain

David E. Hokanson, Joseph M. Laakso, Tianming Lin, E Michael Ostap. Physiology, University of Pennsylvania Sch Med, Philadephia, PA, USA.

Poster 97

A Microtubule-destabilizing Kinesin-13 Motor in Meiosis

Jianwei Zou, PhD, Christine D. Yankel, MS, Kevin Su, Sharyn A. Endow, PhD. Dept Cell Biol, Duke Univ Med Ctr, Durham, NC, USA.

Poster 98

In vivo Measurement of Molecular Motor Forces

George T. Shubeita, PhD, Steven P. Gross. Developmental and Cell Biology, University of California, Irvine, Irvine, CA, USA.

Poster 99

Structural Genomics of Human Kinesin Motors

J Wang, W Tempel, Y Shen, L Shen, A Bochkarev, Hee-Won Park. Structural Genomics Consortium, University of Toronto, Toronto, ON, Canada.

Poster 100

Modeling Molecular Motor: Two-headed Kinesin Study.

Changbong Hyeon, PhD, Jose Nelson Onuchic, PhD. Center for theoretical biological physics, UC San Diego, La Jolla, CA, USA.

Poster 101

Twirling of Myosins II, V and VI

John F. Beausang, M.S.¹, Yuijie Sun, PhD², Yale E. Goldman, MD, PhD². ¹Phyiscs, University of Pennsylvania, Philadelphia, PA, USA, ²Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA, USA.

Poster 102

Walking Mechanism of Kinesin Studied by the Unbinding-Force Measurements

Rumiko Akiyama¹, Yusuke Oguchi¹, Shin'ichi Ishiwata¹,². ¹Department of Physics, Waseda University, Shinjuku-ku, Tokyo, Japan, ²Advanced Research Institute for Science and Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan.

Poster 103

A Myosin V Assay Using Linear Zero-mode Waveguides to Observe Differential Nucleotide Dynamics

L. Stirling Churchman¹, Jonas Korlach², Mathieu Foquet², Michael J. Levene³, James A. Spudich⁴. ¹Department of Physics, Stanford University, Stanford, CA, USA, ²Pacific Biosciences, Menlo Park, CA, USA, ³Department of Biomedical Engineering, Yale University, New Haven, CT, USA, ⁴Department of Biochemistry, Stanford University, Stanford, CA, USA.

Poster 104

Plus End-specific Depolymerase Activity and Plus End-directed Motility of Kip3 (Kinesin-8) Explain Its Role in Positioning Mitotic Spindles During Asymmetric Cell Division

Mohan Gupta¹, Pedro Carvalho¹, David Roof², David Pellman¹. ¹Dana-Farber Cancer Institute, Boston, MA, USA, ²University of Pennsylvania, Philadelphia, PA, USA.