Exhibitor Presentations

Exhibitor Presentations

All Exhibitor Presentations will take place in Room 112 AB of the Pennsylvania Convention Center. Please check back for continuing schedule updates.

Use the following links to jump to presentation descriptions by company: 

Asylum Research
Bruker Corporation
Bruker BioSpin Corporation, EPR Division
FEI Company
ForteBio - A Division of Pall Life Sciences
GE Healthcare
HEKA Elektronik
KinTek Corporation
Molecular Devices, LLC
Nanion Technologies (Presentation 1, Presentation 2)
Nanonics Imaging Ltd. 
SensiQ Technologies, Inc. 
World Precision Instruments
Wyatt Technology Corporation

 

Sunday, February 3

 

GE Healthcare
Sunday, February 3, 9:00 AM - 10:30 AM

Application of ITC, DSC and SPR in biophysical characterization of biomolecules in basic research and in drug discover

Complete biophysical characterization of biopolymers such as proteins involves understanding its structure, stability, function, and activity. The label-free techniques of Isothermal titration calorimetry (ITC) , Surface Plasmon Resonance (SPR) and Differential Scanning Calorimetry (DSC) are used in basic research and drug discovery and development to determine biological processes and molecular recognition, measure binding affinity, binding kinetics, active concentration, stoichiometry, stability and thermodynamics. This information leads to an understanding of structure-function relationships and mechanism of action. This presentation will discuss how ITC, DSC and SPR are used to obtain critical binding and stability-related data, to assist scientists in making decisions during research and development.

 

Bruker Corporation
Sunday, February 3, 11:00 AM - 12:30 PM

Atomic Force Microscopy: A Unique Tool for Probing Mechanical Functions for Biological Process

Physical properties including structures such as shape/size and mechanics such as strength/stiffness/ interaction forces play crucial roles in biological processes. Quantification of this at various length scales is necessary because of the heterogeneous/complex nature of biologics. Atomic force microscopy (AFM) is a unique research tool because of its abilities to perform measurements with both high spatial and force resolution in fluid under physiological conditions. In this tutorial, Bruker will present theories behind AFM, bio-applications in high-speed AFM, and practical guides to quantitative mechanical measurements and analysis of biological samples ranging from a single membrane protein to a single cell. While the key experiments presented will encompass research in microbiology/pain mediation/cancer, the methodology has also been employed in other disciplines including pathogenesis/stem cell differentiation/cell signaling and more.

Presenter: James Shaw

ForteBio - A Division of Pall Life Sciences
Sunday, February 3, 1:00 PM - 2:30 PM

BLItz – Low Volume Protein Characterization and Analysis

The BLItz offers leading label-free detection in as low as 4ul of volume. At a fraction of the price of larger systems, the BLItz is a personal bench top system for identifying and quantifying proteins, monitoring binding kinetics, and simplifying immunoassays. Analyze proteins, antibodies, and other macromolecules in their unmodified states, and speed your research by eliminating unnecessary labeling steps. 

This workshop will include presentation of user data, an overview of applications enabled by the BLItz, and a hands-on demo opportunity. 

Presenter: Craig Tin, Senior Product Manager, ForteBio – A Division of Pall Life Sciences

KinTek Corporation

Sunday, February 3, 3:00 PM - 4:30 PM 
 
New Advances in Fitting Kinetic and Equilibrium Data by Simulation
 
Fitting kinetic data based upon numerical integration of rate equations offers many advantages over conventional fitting of data based upon equations derived from simple models. Fitting by simulation is the most rigorous and eliminates the need to derive equations; however, it also requires and understanding of the kinetics and critical thought to avoid overly complex models.  
 
In this presentation, Dr. Johnson will show how global fitting of kinetic data can be accomplished with ease using the fast, dynamic simulation in KinTek Explorer software, overcoming the all-to-common errors in conventional fitting. Moreover, data are fit to derive rate constants directly defining steps in a model. New advances in the software allow fitting kinetic data from single molecule experiments. In addition, families of curves can be fit simultaneous to define voltage-dependent rate constants or data from Temperature-jump or Pressure-jump experiments.  In addition, equilibrium titration data can be fit using a unique endpoint simulation method and time-resolved spectra can be fit using singular value decomposition. Moreover, all experiments can be fit simultaneously.  
 
Presenters:
Kenneth A. Johnson, Ph.D. President, KinTek Corp.
Roger Williams, Professor of Biochemistry, University of Texas at Austin
 

Asylum Research
Sunday, February 3, 5:00 PM - 6:30 PM

Speed, Ultra-high Resolution Imaging and Cell Mechanics with Atomic Force Microscopy

AFM has improved drastically over the past few years for higher resolution imaging of proteins, nucleic molecules, lipids, as well as for cell and molecular mechanics. For high resolution imaging, AFM technology has made dramatic improvements pioneered by advancements in the Asylum Research Cypher™ AFM. Primarily, miniaturization of cantilevers has increased their resonant frequencies and decreased their thermal noise, allowing faster, lower noise measurements and ultra-high resolution imaging. We'll discuss the science behind these innovations and provide examples of the movement of molecular point defects in bacteriorhodopsin, atomic point defects in calcite, and resolution of the double-helix structure of DNA in solution. 

For cell mechanics, AFMs can be used as mechanical probes, assessing the mechanical properties of cells/tissues which provide novel insights into cell function and cell-substrate interactions. This technique is important to understand for data analyses, and specifically on how model assumptions affect the measured properties. We'll discuss how these models have been newly implemented in our software, and how researchers can easily control and customize their model to their experiment and integrate this information with optical microscopy data. Examples of current cell research performed on the MFP-3D-BIO™ AFM will be presented.

Presenter: Dr. Irene Revenko, Asylum Research

HEKA Elektronik
Sunday, February 3, 7:00 PM - 8:30 PM
 
HEKA Electrophysiology Update
 
For over 40 years, HEKA has consistently updated both hard- and software products and gained a reputation for expert tech support and unmatched customer service.  By popular demand, we are hosting a series of User Meetings with tutorial presentations.  On one hand, we will showcase some of the new products to the experienced user and, on the other hand, we will provide step-by-step guidance to the researcher who is new to the field.
 
The HEKA User Meeting at the Annual Meeting of the Biophysical Society in 2013 will focus on the following topics:
Concurrent imaging and electrophysiology: How to unleash the power of the HEKA Imaging Extension in combination with PATCHMASTER® Software
Product update: How you benefit from new features in HEKA products
Basics in signal conditioning and data processing: How to turn my analog signal into a colored line on the computer screen
 
Who should attend?
- Researchers with experience in patch clamp electrophysiology and related scientific techniques
- Researchers who want to combine imaging applications, such as FURA measurements, with electrophysiology or electrochemistry experiments
- Postdocs and graduate students who want to learn more about electrophysiology techniques
 
Register online at http://heka.eventbrite.com or send an email to marketing@heka.com.  Space, food and drinks are limited and provided on a first-come-first-served basis. So do not delay and register today!  See you in Philadelphia.
 
Presenters:
Jan Dolzer, Vice President, Sales and Marketing, HEKA Worldwide
Telly Galiatsatos, General Manager, HEKA Instruments USA
Hubert Affolter, Softward R&D Lead, HEKA Elektronik

Monday, February 4

Wyatt Technology Corporation
Monday, February 4, 9:00 AM - 10:30 AM

Light Scattering for Label-Free, Immobilization-Free Analysis of Bimolecular Interactions

Wyatt Technology’s Calypso system characterizes bio molecular interactions in solution, without labeling or immobilization, by means of automated Composition-Gradient Multi-Angle static Light Scattering (CG-MALS). CG-MALS is a scientifically rigorous technique that addresses a host of complex self- and hetero-association phenomena, quantifying affinity, stoichiometry, equilibrium and kinetic properties.

Typical uses of the Calypso system include:

• Determining binding affinity and absolute stoichiometry of protein-protein interactions in solution, including multi-domain and multi-protein complexes as well as cooperative binding 
• Analyzing the inhibition or enhancement of protein-protein interactions by small molecules
• Quantifying peptide-protein or RNA-protein binding
• Characterizing how virus-like particles assemble and bind antigens 
• Measuring the loading of protein onto nanoparticle delivery vehicles such as micro gels or liposomes

This seminar is recommended for all scientists who require a more complete picture of the in vitro interactions occurring in the macromolecular systems they study.

Presenter: Dr. Daniel Some

Nanion Technologies
Monday, February 4, 11:00 AM - 12:30 PM

Automated Patch Clamp: From Organelles to Primary Cells, from Single Channels to Action Potentials.  Sophisticated and Easy, Who Says You Can’t Have It All?

The Port-a-Patch represents the smallest patch clamp rig in the world, making patch clamp easy to learn within one hour instead of weeks. The device has extremely versatile add-ons allowing for fast external perfusion, internal perfusion and temperature control, supporting high quality recordings from a multitude of different voltage- and ligand gated ion channels, cell lines, primary cell, organelles and artificial bilayers. 

Recordings from lipid bilayers are highly challenging in terms of bilayer formation, protein reconstitution and signal-to-noise ratio for single-channel recordings. The Orbit facilitates the investigation of bilayer-reconstituted ion channels and nanopores, allowing efficient data generation through 16 parallel recordings. Using Micro Electrode Cavity Array (MECA, Ionera), a 4 x 4 array of circular micro cavities in a highly inert polymer, the bilayer is automatically formed by remotely actuated painting (Ionera- SPREAD). Bilayers can also be formed from giant unilamellar vesicles (GUVs) bursting over the 1 μm aperture contained in a borosilicate glass chip. The GUVs are captured by mild suction, and 16 (4 x 4 array) solvent-free bilayers are formed in parallel. 

Come to our workshop and learn from live, hands-on experiments, how to make high quality recordings from cells and ultra-low noise bilayer recordings from reconstituted ion channels!

Spaces are limited so reserve yours by sending an email to info@nanion.de.

Presenters:
Dr. Andrea Bruggemann, CSO, Nanion Technologies
Dr. Mohamed Krier, Application Scientist, Nanion Technologies
Dr. Gerhard Baaken, Project Ionera, University of Freiburg

Nanonics Imaging Ltd. 
Monday, February 4, 1:00 PM - 2:30 PM

Frequency Modulation BioAFM with Single PicoNewton Forces & Ultrasmall Near-field Illumination Volumes for FCS

BioAFM has been technologically limited and these limitations are repeated in every example of such instruments. There are three such limitations. First, there is the feedback used in every instrument. Second, is the nature of the probe?  Third, are the geometrical constraints in the scanning mechanisms? 

In terms of the feedback, tuning forks address all of the issues that have limited the beam bounce mechanism which is standard in all commercial instruments.  They have ultra high quality characteristics that cannot be reached in any beam bounce silicon cantilever technology. This allows for demonstrated single pN force measurements that are 50-100 times more sensitive than the best techniques based on beam bounce technology and thus open new avenues in Force Spectroscopy for applications such as protein pulling. 

As will be demonstrated a BioAFM with tuning fork feedback also resolves historic problems of using near-field optics on live cells.  This opens ultra-small zeptoliter illumination volumes from a near-field optical probe permitting such measurements as fluorescence correlation spectroscopy on live cells at even micro molar concentration.  This exciting near-field optical application allows for imaging of FCS signals at super-resolution together with the 3D topography of the cell membrane.  Such imaging has never been possible and can also be applied to other fluorescence techniques such as lifetime, FRET etc. or other optical measurements such as Raman. 

In addition to these capabilities it will be shown that the combination of cylindrical probes and tuning forks allow for high Q factors with high force sensitivities and no jump to contact in physiological media.

This portends a new era in BioAFM fully integrated with far-field optical imaging methods and super-resolution optical imaging that will allow this integrated tool to become as ubiquitous as the standard optical microscope in biology.

Presenter: Aaron Lewis, The Hebrew University of Jerusalem, Israel, Nanonics Imaging Ltd.

Bruker BioSpin Corporation, EPR Division
Monday, Feburary 4, 3:00 PM - 4:30 PM
 
EPR from Beer to Deer
 
EPR has become a routine, reliable and invaluable technology with applications from quality control in industry to research in the Biophysics laboratory. We will review the breadth of applications and highlight new advances in both CW and Pulse EPR Spectroscopy.
 
Presenters:
Dr. Peter Hofer, Director of EPR Division, Bruker BioSpin GmbH
Dr. Arthur Heiss, Vice President, Bruker BioSpin Corp.
Dr. Ralph Weber, Sr. Applications Scientist, Bruker BioSpin Corp.
Dr. David Barr, EPR Product Specialist, Bruker BioSpin Corp.

Molecular Devices, LLC
Monday, February 4, 5:00 PM - 6:30 PM

Getting the Most of pCLAMP Software:  Data Acquisition and Analysis

pCLAMP is a powerful data acquisition and analysis software and is widely used for a variety of electrophysiological recordings. In the first tutorial of this workshop, Jeffrey Tang will highlight a few features used to create a customized acquisition protocol in Clampex. These features include Stimulus File, User List, and Sequencing Keys in Clampex. In the second tutorial, Burt Maertz will share tips in single-channel analysis using Clampfit. These include baseline adjustment, filtering, event searching, fitting, and histogram plot.

To RSVP for this free event, visit our online registration page. There is no cost to participate, but space is limited. Upon registration, you will receive an email confirming your registration.

Presenters:
Jeffrey Tang, PhD., Product Marketing Manager of Axon Conventional Electrophysiology, Molecular Devices, LLC
Burt Maertz, Technical Support Specialist of Axon Conventional Electrophysiology, Molecular Devices, LLC

Tuesday, February 5

World Precision Instruments
Tuesday, February 5, 9:00 AM - 10:30 AM

World Precision Instruments Launches Cell Tester: Unique Cell Biology Tool to Sense Stress and Stretch in Individual Cells

The Cell Tester, a novel cell biology research tool, set to revolutionize the emerging field of ‘mechano transduction’ by allowing researchers to study the influence of mechanical force, stress or strain on cells and how these cells react to stimuli.

Many cells in the human body are constantly under the influence of mechanical stresses. Mechanical stretching activates mechano transduction signaling pathways in cells that have broad implications for cell health and disease. Insights into how these cells respond to additional stresses could give researchers valuable insight into disease progression and potentially shed light on how intervention therapies impact on these responses. The ability to do this on single cells opens up the possibility to use live human cells as an alternative to animal models of a disease within the research arena.

Working at a rate of over 1000 measurements per second, the Cell Tester employs state-of-the-art optics, nano positioning and force sensor technology to deliver sensitive, robust and reproducible force measurements. This facilitates the quantification of the very small forces that individual cells can generate – in the order of tens to hundreds of nano grams. At the same time, the instrument uses miniature piezo crystal-based motors to push or pull cells and so changes their length or imposes stress.

“In combination with a laser-scanning confocal microscope, we have been able to follow the fate of calcium release inside heart cells,” remarked Professor W. J. Lederer, Center of Biomedical Engineering and Technology, University of Maryland School of Medicine. “Being able to gain insight into the relationship between mechanical stress and aberrant calcium release, thought to cause possible lethal arrhythmias, will help support the discovery of new targets for the treatment of heart disease.”

Presenter: Dr. Harm Knot

Nanion Technologies
Tuesday, February 5, 11:00 AM - 12:30 PM

SURFE2 – Catch the Wave for Transporters.  Precise Measurements of Membrane Transporter Protein Activity

Ion transporters and pumps play an important role within general metabolism and information processing of organisms. Dysfunction and -regulation of transporter proteins are related to diseases like obesity, diabetes, hypertension, and CNS disorders such as epilepsy and depression. Hence, ion transporters become potential targets within the drug development treating disease-related abnormalities. At the present, labeling technologies and conventional patch clamp are commonly used for ion transporter screening. However, radioactive and fluorescence-based assays have limited sensitivity, and because of the limited molecule turnover per seconds of transporters and pumps compared to ion channels, the direct electrophysiological measurement of protein transporters and pumps activity is highly challenging.

Here, we present the SURFE2R technology – an easy-to-handle, highly sensitive and highly efficient screening platform for direct measurements of ion transporters and ion channels in diverse and heterologous membranes. The SURFER2R technology was established and developed by IonGate/Scientific Devices Heidelberg. Nanion continues with the SURFE2R product line with on-going development of high throughput systems, which will be presented in this workshop. 

The SURFE2R N1 is a small footprint, fully automated device recording from membrane preparations, with proven success using native tissue, mammalian and insect cell lines, bacteria, organelles, and proteoliposomes. 

Catch the Wave, come to our workshop and learn from LIVE-experiments how to measure transporter-protein functionality, efficient and reliable. 

Spaces are limited so reserve yours by sending an email to info@nanion.de.

Presenters: 
Dr. Andrea Bruggemann, CSO, Nanion Technologies
Maria Barthmes, PhD student, Nanion Technologies

SensiQ Technologies, Inc. 
Tuesday, February 5, 1:00 PM - 2:30 PM

Label-free screening using compound titrations for increased throughput and data quality

A recently introduced compound titration technique, OneStepTM, has been shown to provide several benefits over standard fixed concentration injections (FCI) including; higher throughput with higher resolution; enhanced resolution of multisite binding; improved tolerance to promiscuous binding; and, higher tolerance to target degradation. Further, because the titration is generated by a hydrodynamic effect, the compounds’ diffusion coefficient can also be estimated. It is then possible to identify aggregation artifacts such as micelle formation by comparing fitted diffusion coefficients with those expected from theory. In the current work we extend the theory and practice to accommodate cases where compound reversibly interacts with the tubing wall. This retention factor increases the residence time of the compound relative to the solvent and provides a solubility ranking along with the kinetic/affinity analysis. Additionally, the titration can be performed at high flow rates for rapid primary screening to determine the affinity constant which avoids the usual follow up analysis and saves considerable time and effort.

Presenter:
Mr. Aaron Martin


FEI Company
Tuesday, February 5, 3:00 PM - 4:30 PM

Tecnai Arctica™: The way to answer real biological questions through unraveling the 3D mechanistics of protein machineries

Fundamental research within the scope of structural cell biology is increasingly focusing on unraveling interactive biological and biochemical processes and pathways at the macromolecular level. Of paramount importance is the three-dimensional visualization of macromolecular structures and molecular machines in their native functional state. Three techniques play a major role in orchestrating this.

Nuclear magnetic resonance (NMR) has the capability to unravel specific protein domains or fragments and their functional role in protein binding and folding whereas X-Ray Crystallography (XRD) allows visualizing 3D structures of mainly monomer and dimer origin after crystallization. To unravel the fundamental biological challenges however, it is essential to visualize multimeric complexes in their tertiary and quaternary state and their interaction with other complexes. By performing typical cryo-TEM applications like single particle analysis or tomography, this can be achieved. In this so-called translation methodology, NMR, XRD and cryo-TEM thus provide complementary information in order to resolve the real biological questions.

FEI has developed a unique workflow solution around the main applications for cryo-TEM (i.e. single particle analysis and tomography) that comprises of a method for automated sample preparation, image recording and data analysis. Thus, the FEI solution contributes to an improved time to high quality data and consequently reduced cost per structure. With this aim FEI is proud to introduce the Tecnai Arctica™, the next generation and most powerful and flexible high resolution electron microscope for 3D characterization of biological samples.

In this conference presentation, the various components of the unique FEI workflow solution are presented and exemplified by a number of applications. 

Presenters: 
Marc Storms
Raymond Wagner
Gijs van Duinen
Matthijn Vos
Kasim Sader
Thomas Wohlfarth
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