All Exhibitor Presentations will take place in Room 22 of the San Diego Convention Center. Please check back for continuing schedule updates.
Sunday, February 26
11:00 AM - 12:30 PM: Bruker
A New Generation of Bruker AFMs for Biophysicists
In this tutorial Bruker will present the latest advances in their full-line of atomic force microscopes (AFM) that continue to put their AFM systems at the forefront of biophysical research. Data will be presented from various application areas demonstrating the use of the self-optimizing ScanAsyst® Mode for ultra-low force high-resolution imaging of soft biological samples and PeakForce QNM® for correlative spatial mapping of sample nano-mechanical properties. We will present the latest in functionally integrated AFM and optical microscopy for live cell studies. We will also discuss how Bruker’s advances in high-speed AFM imaging are improving research productivity and providing biophysicists with unprecedented opportunities to directly observe the dynamics of biologically-related processes.
1:00 - 2:30 PM: Nanonics Imaging
A Frequency Modulation Tuning Fork Based Multiprobe BioAFM.
Aaron Lewis, The Selim and Rachel Banin School of Computer Science & Engineering, The Hebrew University of Jerusalem, Israel
In this presentation, Nanonics Imaging introduces the HydraTMMultiprobeBioAFM system, a revolutionary BioAFM, allowing for unprecedented new levels in sensitivity and control in BioAFM imaging. Based on a Tuning Fork Feedback Mechanism with an open optical axis, this system permits the ultimate in AFM sensitivity, and full integration with standard upright, inverted or dual optical microscopes.
We will show how tuning forks and ultra-high quality factor probes address the quality issues that have limited BioAFM’s. This allows for ultrasmall force induced frequency changes to be detected with a sensitivity 100 times better than standard beam bounce-based AFM systems, opening new avenues in Force Spectroscopy for applications such as protein pulling. Tuning Fork Feedback also resolves problems such as jump to contact and adhesion ringing. Completely transparent feedback provides transparent optical and geometrical coupling with all microscopes including Raman, fluorescence, FRET, FCS, etc. and introduces a multitude of new integrations and applications.
3:00 - 4:30 PM: Carl Zeiss Microscopy
Scientific Advances Through New Microscopy: The Helium Ion Microscope
Larry Scipioni, Carl Zeiss Microscopy
The Helium Ion Microscope (HIM) has been described as an impact technology, offering new windows into nanoscale imaging. Combining a high brightness Gas Field Ion Source (GFIS) with unique sample interaction dynamics, the HIM provides images offering unique contrast and high resolution for imaging of biological and bioengineering specimens. This talk will highlight the capabilities of the instrument and show examples of active research in biology that is being conducted with this technology. The superior resolution and surface sensitivity enhances the ability to view processes on membranes and other surfaces. The unique charge neutralization technology, not possible with SEM, allow capturing images with sub-nanometer resolution even on samples that have no conductive coating. This brings the imaging closer to representing the true state of the specimen under study.
5:00 - 6:30 PM: Asylum Research
Smaller, Quieter, Faster Atomic Force Microscopy
Recently, cantilevers as small as 10µm and with resonant frequencies >5 MHz have become readily available. Atomic Force Microscopes such as the Cypher AFM from Asylum Research have been optimized for use with these cantilevers, bringing major new benefits to AFM. The first benefit is much smaller thermal noise, enabling quieter force measurements and higher resolution imaging with Angstrom-scale cantilever amplitudes. The second advance is a major speed boost for AC modes (tapping, non-contact) in both air and liquid. When coupled with other instrumental improvements such as a high-speed scanner, the shorter levers allow scanning with good tracking at rates 20 to 40 times those possible with conventional levers. We'll discuss the technical details behind both these improvements and present images and movies highlighting the improvements, including images showing individual vacancy defects on crystals and movies showing fast scanning on polymers, crystals, and biological samples including bacteriorhodopsin and DNA.
7:00 - 8:30 PM: Cytocentrics Bioscience GmbH
CytoPatchTM: An Easy-To-Use Automated Single Cell Patch Clamp System That Matches Manual Patch Clamp Data Quality.
Graeme Daniels, Cytocentrics Bioscience GmbH
Olaf Scheel, Cytocentrics Bioscience GmbH
The heart of the CytoPatch™ Instrument is the patented CytoPatch™ Chip. Its unique design incorporates a glass pipette which mimics the properties of the conventional patch clamp electrode. The CytoPatch™ Chip is the only automated patch clamp system that has two access channels, allowing robust gigaseal recordings lasting over 30 minutes coupled with exceptional perfusion control. This presentation and hands-on demonstration discusses the system and some of the data that users have produced using it.
Monday, February 27
9:00 - 10:30 AM: Nanosurf, Inc.
FluidFM Technology – Opening New Avenues for Single Cell Biology
FluidFM is a new technology that combines a force controlled micromanipulator (AFM technology) via a hollow cantilever with microfluidics. Cantilevers fashioned with a tiny opening at their free end can be used for various single cell manipulations including: pick & place, adhesion, injection, extraction, and electrophysiology. Due to its versatility, minimal invasive nature, automation capabilities and its relative ease of use, the technology allows single cell experiments at much higher throughput and is expected to play a significant role in areas like proteomics, metabolomics, drug discovery, cell mechanics and stem cell research. In this presentation, we will discuss recent developments in FluidFM single cell applications.
11:00 AM - 12:30 PM: Nanion Technologies
Automated patch clamp: from organelles to primary cells, from single channels to action potentials. Sophisticated and easy, who says you can’t have it all?
Andrea Bruggemann, CSO of Nanion
Mohamed Kreir, Application Scientist.
The Port-a-Patch is a highly versatile, easy-to-use patch clamp platform, supporting high quality recordings from a multitude of different voltage- and ligand gated ion channels, cell lines, primary cell, organelles and artificial bilayers. Come to our workshop and learn from live, hands-on experiments, how to make high quality recordings from cells and ultra-low noise bilayer recordings from reconstituted ion channels! Spaces are limited so reserve yours by sending an email to firstname.lastname@example.org.
1:00 - 2:30 PM: SensiQ
Enhanced SPR Analysis Techniques
John Quinn, PhD Chief Scientist, SensiQ
Dr Quinn will describe new SPR injection techniques that allow enhanced binding analysis of complex analytes. The new Taylor Dispersion Injection technique will be described which allows rapid kinetic analysis of multisite binding with a single injection. The Fast-step(TM) injection technique will also be described as a method to obtain rapid concentration analysis with only one injection.
3:00 - 4:30 PM: Fluxion Biosciences
IonFlux System: Automated Patch Clamp Redefined
Stephen Smith, Ph.D.
Technical Director, Fluxion Biosciences
See how IonFlux System is changing the field of ion channel drug discovery and research. In this presentation, you will learn how the IonFlux can be utilized for a variety of ion channel applications. The IonFlux System features continuous compound perfusion, unmatched protocol flexibility, and a modular benchtop architecture. The basic principles of operation will be reviewed along with representative data sets and performance specifications. Key benefits of the system include: suitability for both voltage- and ligand-gated channels, highest available throughput, lowest running cost, simple plate reader format, and assay flexibility. A research version is also available for about the cost of a manual patch rig.
5:00 - 6:30 PM: Molecular Devices, Inc.
Getting the Most out of pCLAMP Software: Data Acquisition and Analysis Tutorials
Jeffrey Tang, Product Marketing Manager of Axon Conventional Electrophysiology, Molecular Devices, LLC.
Burt Maertz, Technical Support Specialist of Axon Conventional Electrophysiology, Molecular Devices, LLC.
pCLAMP is a powerful data acquisition and analysis software and is widely used for a variety of electrophysiological recordings. In the first tutorial of this workshop, Jeffrey Tang will walk through the Protocol Editor in Clampex. In his presentation he will cover: 1) how to select appropriate data acquisition mode for your experiments; 2) how to set up protocols for voltage-clamp and current-clamp recordings; 3) how to execute simple arithmetic calculations on two acquired signals; 4) how to create your own stimulus waveform or import stimulus waveform from data file; 5) how to perform pre-sweep trains and P/N leak subtraction; and 6) how to perform User List and membrane test between sweep. In the second tutorial, Burt Maertz will share tips on data conditioning and analysis in Clampfit. These include baseline adjustment, off-line filtering, power spectrum and arithmetic calculation. To register for this free event, please email Jeffrey Tang
. There is no cost to participate, but space is limited. Upon registration, you will receive an email confirming your registration.
Tuesday, February 28
9:00 AM - 10:30 AM: Applied Precision, Inc. (API)
Advances in Live-Cell Super-Resolution Imaging – Applied Precision, A GE Healthcare Company
3D super-resolution microscopy represents a new approach that is challenging our understanding of the inner workings of the cell. The ability to see the inner structure of the cell, at resolutions previously unobtainable, is enabling a new era of discovery in biological science. Now Applied Precision is proud to push this envelope further by introducing the OMX V4 platform and the revolutionary OMX Blaze Live Cell Super-Resolution System. Come join us at our presentation to learn about:
• OMX V4 System and approaches to super-resolution imaging
• Live-cell super-resolution imaging
• New approach localization microscopy with Monet™
• Latest research using OMX technology
11:00 AM - 12:30 PM: SDx Tethered Membranes
Bruce Cornell will demonstrate stable, ready-to-use bilayer membranes tethered to patterned gold electrodes in a six well flow cell cartridge, tethaPlate™ and the SDxTM tethaPod™, a plug-and-play conductance and capacitance reader. He will discuss how to tailor lipid densities and spacer lengths to allow ionic conduction measurement of medium molecular weight ion channels such as VDAC, NaChBac, mechanosensitive channels, hemolysin, chloride channels, and Kv channels.
1:00 - 2:30 PM: Thomson Reuters
Advance Your Research, Create Bibliographies Instantly
Paul Dunlap, Thomson Reuters
Discover how EndNote can save you valuable time managing your reference collection, simplifying collaboration with colleagues and increasing accuracy in cited papers. If you already use EndNote, see new features in action that expand PDF and file attachment management to the Web, and combine searching, citing, and bibliography creation into one streamlined process that results in submission-ready manuscripts. In this session you’ll see how EndNote X5 makes it easy to collect references and full text PDF files, organize your references into groups, and share your research with others using your EndNote Web account. You will learn about new options available in Cite While You Write™ for field substitution and more. We will discuss easy ways to collaborate with integrated solutions provided by Thomson Reuters: EndNote; Web of Knowledge, a premiere research platform; and ResearcherID, a free author community where authors are identified uniquely. Your questions are welcome at this interactive session.
3:00 PM - 4:30 PM: KinTek Corporation
New Advances in Fitting Kinetic Data by Simulation: From Single molecules to Voltage- or Temperature-dependent Rate Constants
Kenneth A. Johnson, Ph.D. President, KinTek Corporation.
Roger Williams Professor of Biochemistry, University of Texas at Austin
Fitting kinetic data based upon numerical integration of rate equations offers many advantages over conventional fitting of data based upon equations derived from simple models. In this presentation, Dr. Johnson will show how global fitting of kinetic data can be accomplished with ease using the fast, dynamic simulation in KinTek Explorer software, overcoming the all-to-common errors in conventional fitting. Moreover, data are fit to derive rate constants directly defining steps in a model. New advances in the software allow fitting kinetic data from single molecule experiments. In addition, families of curves can be fit simultaneous to define voltage-dependent rate constants. Similarly, one can fit data from Temperature-jump or Pressure-jump experiments.